PXD017523

PXD017523 is an original dataset announced via ProteomeXchange.

Title	Rab28 is localised to photoreceptor outer segments, regulates outer segment shedding but is not linked to retinal degeneration in zebrafish
Description	The photoreceptor outer segment is the canonical example of a modified and highly specialised cilium, with an expanded membrane surface area in the form of discs or lamellae for efficient light detection. Many ciliary proteins are essential for normal photoreceptor function and cilium dysfunction often results in retinal degeneration leading to impaired vision. Herein, we investigate the function and localisation of the ciliary G-protein RAB28 in zebrafish cone photoreceptors. CRISPR-Cas9 generated rab28 mutant zebrafish display a reduction in shed outer segment material in the RPE at 1 month post fertilisation (mpf), but otherwise normal retinal structure and visual function up to 12 mpf. Cone photoreceptorspecific transgenic reporter lines show Rab28 localises almost exclusively to outer segments, independently of nucleotide binding. Co-immunoprecipitation analysis demonstrates tagged Rab28 interacts with components of the phototransduction cascade, including opsins, Phosphodiesterase 6C and Guanylate Cyclase 2D. Our data shed light on RAB28 function in cones and provide a model for RAB28-associated cone-rod dystrophy
HostingRepository	PRIDE
AnnounceDate	2020-04-09
AnnouncementXML	Submission_2020-04-09_06:54:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Matallanas
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-14 08:54:56	ID requested
⏵ 1	2020-04-09 06:54:16	announced

Carter SP, Moran AL, Matallanas D, McManus GJ, Blacque OE, Kennedy BN, Genetic Deletion of Zebrafish Rab28 Causes Defective Outer Segment Shedding, but Not Retinal Degeneration. Front Cell Dev Biol, 8():136(2020) [pubmed]

submitter keyword: Zebrafish, retinal degeneration, Rab28, RPE, phototransduction cascade, cone-rod dystrophy

Brendan Kennedy
contact affiliation	UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland.
contact email	brendan.kennedy@ucd.ie
lab head
David Matallanas
contact affiliation	Systems Biology Ireland, University College Dublin
contact email	david.gomez@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017523
     1. Label: PRIDE project
     2. Name: Rab28 is localised to photoreceptor outer segments, regulates outer segment shedding but is not linked to retinal degeneration in zebrafish