PXD017513
PXD017513 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Serial In-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis |
Description | Advancement in mass spectrometry has revolutionised the field of proteomics. However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation. Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions. Of particular interest is the brain extracellular matrix (ECM), which has been called the “final frontier” in neuroscience that consists of highly glycosylated proteins. Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) and form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases. Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture. The protocol presented here integrates glycomics and proteomics to analyze multiple classes of biomolecules. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:03:04.186.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD017513 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Manveen Sethi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-02-14 01:27:35 | ID requested | |
1 | 2020-04-29 23:21:46 | announced | |
⏵ 2 | 2024-10-22 05:03:05 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1039/d0mo00019a; |
10.6019/PXD017513; |
Sethi MK, Downs M, Zaia J, Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis. Mol Omics, 16(4):364-376(2020) [pubmed] |
Keyword List
submitter keyword: Glycosaminoglycans, proteomics, mass spectrometry,Glycomics, proteoglycans, FASP, extracellular matrix. |
Contact List
Prof. Joseph Zaia | |
---|---|
contact affiliation | Center for biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston University, Boston MA 02125 |
contact email | jzaia@bu.edu |
lab head | |
Manveen Sethi | |
contact affiliation | Boston University |
contact email | msethi@bu.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017513 |
PRIDE project URI |
Repository Record List
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