PXD017513

PXD017513 is an original dataset announced via ProteomeXchange.

Title	Serial In-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis
Description	Advancement in mass spectrometry has revolutionised the field of proteomics. However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation. Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions. Of particular interest is the brain extracellular matrix (ECM), which has been called the “final frontier” in neuroscience that consists of highly glycosylated proteins. Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) and form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases. Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture. The protocol presented here integrates glycomics and proteomics to analyze multiple classes of biomolecules.
HostingRepository	PRIDE
AnnounceDate	2020-04-30
AnnouncementXML	Submission_2020-04-29_23:21:44.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017513
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Manveen Sethi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-14 01:27:35	ID requested
⏵ 1	2020-04-29 23:21:46	announced

Sethi MK, Downs M, Zaia J, Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis. Mol Omics, 16(4):364-376(2020) [pubmed]

submitter keyword: Glycomics, proteomics, mass spectrometry, FASP, Glycosaminoglycans, proteoglycans, extracellular matrix.

Prof. Joseph Zaia
contact affiliation	Center for biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston University, Boston MA 02125
contact email	jzaia@bu.edu
lab head
Manveen Sethi
contact affiliation	Boston University
contact email	msethi@bu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017513
     1. Label: PRIDE project
     2. Name: Serial In-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis