PXD017511

PXD017511 is an original dataset announced via ProteomeXchange.

Title	Identification of Novel Lipid Droplet Factors that Regulate Lipophagy and Cholesterol Efflux in Macrophage Foam Cells
Description	Macrophage autophagy is a highly anti-atherogenic process that promotes the catabolism of cytosolic lipid droplets (LDs) to maintain cellular lipid homeostasis. Selective autophagy relies on tags such as ubiquitin and a set of selectivity factors including selective autophagy receptors (SARs) to label specific cargo for degradation. Originally described in yeast cells, ‘lipophagy’ refers to the degradation of LDs by autophagy. Yet, how LDs are targeted for autophagy is poorly defined. Here, we employed mass spectrometry to identify lipophagy factors within the macrophage foam cell LD proteome. In addition to structural proteins (e.g. PLIN2), metabolic enzymes (e.g. ACSL) and neutral lipases (e.g. PLPL2), we found the association of proteins related to the ubiquitination machinery (e.g. AUP1) and autophagy (e.g. HMGB, 1433).
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:54:07.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathieu Lavallée-Adam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-14 01:27:12	ID requested
⏵ 1	2022-02-15 09:54:08	announced

Robichaud S, Fairman G, Vijithakumar V, Mak E, Cook DP, Pelletier AR, Huard S, Vanderhyden BC, Figeys D, Lavall, é, e-Adam M, Baetz K, Ouimet M, Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells. Autophagy, 17(11):3671-3689(2021) [pubmed]

submitter keyword: Human, Macrophage foam cell, Lipid Droplet, LC-MS/MS

Mireille Ouimet
contact affiliation	University of Ottawa
contact email	mireille.ouimet@uottawa.ca
lab head
Mathieu Lavallée-Adam
contact affiliation	University of Ottawa
contact email	mathieu.lavallee@uottawa.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD017511

PRIDE project URI

• PRIDE
  1. PXD017511
     1. Label: PRIDE project
     2. Name: Identification of Novel Lipid Droplet Factors that Regulate Lipophagy and Cholesterol Efflux in Macrophage Foam Cells