PXD017485

PXD017485 is an original dataset announced via ProteomeXchange.

Title	A GMC Oxidoreductase GmcA is required for symbiotic nitrogen fixation in Rhizobium leguminosarum bv. Viciae
Description	GmcA is a FAD-containing enzyme belonging to the GMC (glucose-methanol-choline oxidase) family of oxidoreductases. A mutation in the R. leguminosarum gmcA gene was generated by homologous recombination. The mutation in gmcA did not affect the growth of R. leguminosarum, but it displayed decreased antioxidative capacity at H2O2 conditions higher than 5 mM. The gmcA mutant strain displayed no difference of glutathione reductase activity, but significantly lower level of the glutathione peroxidase activity than the wild type. Although the gmcA mutant can induce the formation of nodules, the symbiotic ability was severely impaired, which led to an abnormal nodulation phenotype coupled to a 30% reduction in the nitrogen fixation capacity. The observation on ultrastructure of 4-week pea nodules showed that the mutant bacteroids tended to start senescence earlier, and accumulate poly-β-hydroxybutyrate (PHB) granules. In addition, the gmcA mutant was severely impaired in rhizosphere colonization. Real-time quantitative PCR showed that the gmcA gene expression is significantly upregulated in all the detected stages of nodule development, and statistically significant decreases in the expression of the redoxin genes katG, katE and ohrB, were found in gmcA mutant bacteroids. LC-MS/MS analysis quantitative proteomics techniques were employed to compare differential gmcA mutant root bacteroids in response to the wild type infection. Sixty differentially expressed proteins were identified including 33 up-regulated and 27 down-regulated proteins. By sorting the identified proteins according to metabolic function, fifteen proteins were transporter protein, twelve proteins were related to stress response and virulence, and nine proteins were related to transcription factor activity. Moreover, nine proteins related to amino acid metabolism were over-expressed.
HostingRepository	PRIDE
AnnounceDate	2020-02-24
AnnouncementXML	Submission_2020-04-30_03:31:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guojun Cheng
SpeciesList	scientific name: Rhizobium leguminosarum bv. viciae; NCBI TaxID: 387;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 05:37:35	ID requested
1	2020-02-24 00:30:05	announced
⏵ 2	2020-04-30 03:32:06	announced	2020-04-30: Updated publication reference for PubMed record(s): 32265862.

Zou Q, Luo S, Wu H, He D, Li X, Cheng G, . Front Microbiol, 11():394(2020) [pubmed]

submitter keyword: Rhizobium leguminosarum

the glucose-methanol-choline oxidoreductase GmcA

symbiotic nitrogen fixation

antioxidant and symbiotic gene expression

quantitative proteomics

Guojun Cheng
contact affiliation	College of Life Sciences, South-Central University for Nationalities
contact email	chengguojun@mail.scuec.edu.cn
lab head
Guojun Cheng
contact affiliation	College of Life Sciences, South-Central University for Nationalities
contact email	chengguojun@mail.scuec.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017485
     1. Label: PRIDE project
     2. Name: A GMC Oxidoreductase GmcA is required for symbiotic nitrogen fixation in Rhizobium leguminosarum bv. Viciae