PXD017482 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A High Resolution Mass Spectrometry Study Reveals the Potential of Disulfide Formation in Human Mitochondrial Voltage-Dependent Anion Selective Channel Isoforms (hVDACs) |
Description | The voltage-dependent anion-selective channels (VDACs), also known as eukaryotic porins, are pore-forming proteins which allow the passage of ions and small molecules across the outer mitochondrial membrane (OMM). They are involved in complex interactions regulating organelle and cellular metabolism. We have recently reported about the post-translational modifications (PTMs) of the three VDAC isoforms purified from rat liver mitochondria (rVDACs), showing for the first time the over-oxidation of the cysteine residues as an exclusive feature of VDACs. Noteworthy, this peculiar PTM is not detectable in other integral membrane mitochondrial proteins, as defined by their elution at low salt concentration by a hydroxyapatite column. In this study, the association of tryptic and chymotryptic proteolysis with UHPLC/High Resolution nESI-MS/MS, allowed us to extend the investigation to the human VDACs. Over-oxidation of the cysteine residues, essentially irreversible in cell conditions, was as certained also in VDAC isoforms from human cells. In human VDAC2 and 3 isoforms the permanently reduced state of a cluster of close cysteines indicates the possibility that disulfide bridges are formed in the proteins. Importantly, the detailed oxidative PTMs found in human VDACs confirm and sustain our previous findings in rat tissues, claiming for a predictable characterization that has to be conveyed in the functional role of VDAC proteins within the cell. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:00:02.147.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rosaria Saletti |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-12 03:16:04 | ID requested | |
1 | 2020-03-09 00:40:10 | announced | |
⏵ 2 | 2024-10-22 05:00:05 | announced | 2024-10-22: Updated project metadata. |
Publication List
Pittal, à MGG, Saletti R, Reina S, Cunsolo V, De Pinto V, Foti S, A High Resolution Mass Spectrometry Study Reveals the Potential of Disulfide Formation in Human Mitochondrial Voltage-Dependent Anion Selective Channel Isoforms (hVDACs). Int J Mol Sci, 21(4):(2020) [pubmed] |
10.3390/ijms21041468; |
Keyword List
submitter keyword: Cysteine over-oxidation |
mitochondria |
Orbitrap Fusion Tribrid |
hydroxyapatite |
mitochondrial intermembrane space |
outer mitochondrial membrane |
post-translational modification |
Contact List
Salvatore Foti |
contact affiliation | Organic Mass Spectrometry Laboratory, Department of Chemical Sciences, University of Catania |
contact email | sfoti@unict.it |
lab head | |
Rosaria Saletti |
contact affiliation | University of Catania |
contact email | rsaletti@unict.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017482
- Label: PRIDE project
- Name: A High Resolution Mass Spectrometry Study Reveals the Potential of Disulfide Formation in Human Mitochondrial Voltage-Dependent Anion Selective Channel Isoforms (hVDACs)