PXD017478

PXD017478 is an original dataset announced via ProteomeXchange.

Title	Filling the gaps – a call for comprehensive analysis of extracellular matrix of the glial scar in region- and injury-specific contexts
Description	CNS injury results in chronic scar formation that interferes with function and inhibits repair. Extracellular matrix (ECM) is prominent in the scar and potently regulates cell behavior. However, comprehensive information about the ECM proteome is largely lacking, and region- as well as injury-specific differences are often not taken into account. These aspects are the focus of our perspective on injury and scar formation. To highlight the importance of such comprehensive proteome analysis we include data obtained with novel analysis tools of the ECM composition in the scar and show the contribution of monocytes to the ECM composition after traumatic brain injury (TBI). Monocyte invasion was reduced using the CCR2-/- mouse line and step-wise de-cellularization and proteomics allowed determining monocyte-dependent ECM composition and architecture of the glial scar. We find significant reduction in the ECM proteins Tgm1, Itih (1,2, and 3), and Ftl in the absence of monocyte invasion. We also describe the scar ECM comprising zones with distinctive composition and show a subacute signature upon comparison to proteome obtained at earlier times after TBI. These results are discussed in light of injury-, region- and time-specific regulation of scar formation highlighting the urgent need to differentiate injury conditions and CNS-regions using comprehensive ECM analysis.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-27_03:30:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacob Kjell
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 03:03:43	ID requested
⏵ 1	2020-05-27 03:30:26	announced

Kjell J, G, ö, tz M, Filling the Gaps - A Call for Comprehensive Analysis of Extracellular Matrix of the Glial Scar in Region- and Injury-Specific Contexts. Front Cell Neurosci, 14():32(2020) [pubmed]

submitter keyword: Brain injury, extracellular matrix, macrophages, gliosis, scarring, central nervous system

Magdalena Götz
contact affiliation	LMU Muenchen
contact email	magdalena.goetz@helmholtz-muenchen.de
lab head
Jacob Kjell
contact affiliation	Karolinska Institutet
contact email	jacob.kjell@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017478

PRIDE project URI

• PRIDE
  1. PXD017478
     1. Label: PRIDE project
     2. Name: Filling the gaps – a call for comprehensive analysis of extracellular matrix of the glial scar in region- and injury-specific contexts