PXD017475

PXD017475 is an original dataset announced via ProteomeXchange.

Title	The lncRNA MIR31HG regulates the senescence associated secretory phenotype
Description	Oncogene-induced senescence provides a barrier against malignant transformation. Senescent cells secrete pro-inflammatory cytokines, chemokines and growth factors collectively known as the senescence-associated secretory phenotype (SASP). Paradoxically, the SASP can also negatively impact the neighbouring tissues through inducing an inflammatory environment that promotes tumorigenesis and aged-related pathologies. We have previously shown that the lncRNA MIR31HG is overexpressed and located in the cytoplasm during BRAF-induced senescence. In young proliferating cells, nuclear MIR31HG inhibits p16/CDKN2A expression through interaction with polycomb repressor complexes (PRC1/2). Here, we show that MIR31HG regulates the expression and secretion of a subset of SASP components during BRAF-induced senescence. The SASP secreted from senescent cells depleted for MIR31HG fails to induce paracrine invasion without affecting the growth inhibitory effect. Mechanistically, MIR31HG interacts with the translation factor YBX1 facilitating its phosphorylation at serine 102 (p-YBX1S102) by the kinase RSK. p-YBX1S102 induces IL1A translation which acts as upstream regulator inducing the transcription of the other SASP mRNAs. Our results suggest a dual role for MIR31HG in senescence depending on its cellular localization and place the lncRNA as a potential therapeutic target in the treatment of senescence-related pathologies.
HostingRepository	PRIDE
AnnounceDate	2021-03-02
AnnouncementXML	Submission_2021-03-02_07:59:42.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Lubas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 03:02:52	ID requested
⏵ 1	2021-03-02 07:59:43	announced

Dataset with its publication pending

submitter keyword: lncRNA senescence MIR31HG YBX1 SASP BRAF

Anders Lund
contact affiliation	Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen 2200, Denmark
contact email	anders.lund@bric.ku.dk
lab head
Michal Lubas
contact affiliation	University of Southern Denmark
contact email	mlubas@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017475
     1. Label: PRIDE project
     2. Name: The lncRNA MIR31HG regulates the senescence associated secretory phenotype