PXD017470 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Distinct gene clusters drive formation of ferrosome organelles in bacteria |
Description | Cellular iron homeostasis is vital and maintained through tight regulation of iron import, efflux, storage and detoxification1-3. The most common modes of iron storage use proteinaceous compartments, such as ferritins and related proteins4,5. Although lipid-bounded iron compartments have also been described, the basis for their formation and function remains unknown6,7. Here we focus on one such compartment, herein named the 'ferrosome', that was previously observed in the anaerobic bacterium Desulfovibrio magneticus6. Using a proteomic approach, we identify three ferrosome-associated (Fez) proteins that are responsible for forming ferrosomes in D. magneticus. Fez proteins are encoded in a putative operon and include FezB, a P1B-6-ATPase found in phylogenetically and metabolically diverse species of bacteria and archaea. We show that two other bacterial species, Rhodopseudomonas palustris and Shewanella putrefaciens, make ferrosomes through the action of their six-gene fez operon. Additionally, we find that fez operons are sufficient for ferrosome formation in foreign hosts. Using S. putrefaciens as a model, we show that ferrosomes probably have a role in the anaerobic adaptation to iron starvation. Overall, this work establishes ferrosomes as a new class of iron storage organelles and sets the stage for studying their formation and structure in diverse microorganisms. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:37:20.847.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Carly Grant |
SpeciesList | scientific name: Desulfovibrio magneticus RS-1; NCBI TaxID: 573370; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt G2-S HDMS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-12 03:00:42 | ID requested | |
1 | 2022-05-31 03:33:10 | announced | |
⏵ 2 | 2024-10-22 05:37:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41586-022-04741-x; |
Grant CR, Amor M, Trujillo HA, Krishnapura S, Iavarone AT, Komeili A, Distinct gene clusters drive formation of ferrosome organelles in bacteria. Nature, 606(7912):160-164(2022) [pubmed] |
Keyword List
submitter keyword: anaerobic,bacteria, organelle, iron |
Contact List
Arash Komeili |
contact affiliation | Department of Plant and Microbial Biology, University of California, Berkeley, USA |
contact email | komeili@berkeley.edu |
lab head | |
Carly Grant |
contact affiliation | University of California, Berkeley |
contact email | carly.grant@berkeley.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017470
- Label: PRIDE project
- Name: Distinct gene clusters drive formation of ferrosome organelles in bacteria