PXD017470

PXD017470 is an original dataset announced via ProteomeXchange.

Title	Distinct gene clusters drive formation of ferrosome organelles in bacteria
Description	Cellular iron homeostasis is vital and maintained through tight regulation of iron import, efflux, storage and detoxification1-3. The most common modes of iron storage use proteinaceous compartments, such as ferritins and related proteins4,5. Although lipid-bounded iron compartments have also been described, the basis for their formation and function remains unknown6,7. Here we focus on one such compartment, herein named the 'ferrosome', that was previously observed in the anaerobic bacterium Desulfovibrio magneticus6. Using a proteomic approach, we identify three ferrosome-associated (Fez) proteins that are responsible for forming ferrosomes in D. magneticus. Fez proteins are encoded in a putative operon and include FezB, a P1B-6-ATPase found in phylogenetically and metabolically diverse species of bacteria and archaea. We show that two other bacterial species, Rhodopseudomonas palustris and Shewanella putrefaciens, make ferrosomes through the action of their six-gene fez operon. Additionally, we find that fez operons are sufficient for ferrosome formation in foreign hosts. Using S. putrefaciens as a model, we show that ferrosomes probably have a role in the anaerobic adaptation to iron starvation. Overall, this work establishes ferrosomes as a new class of iron storage organelles and sets the stage for studying their formation and structure in diverse microorganisms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:37:20.847.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carly Grant
SpeciesList	scientific name: Desulfovibrio magneticus RS-1; NCBI TaxID: 573370; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt G2-S HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 03:00:42	ID requested
1	2022-05-31 03:33:10	announced
⏵ 2	2024-10-22 05:37:21	announced	2024-10-22: Updated project metadata.

10.1038/s41586-022-04741-x;

Grant CR, Amor M, Trujillo HA, Krishnapura S, Iavarone AT, Komeili A, Distinct gene clusters drive formation of ferrosome organelles in bacteria. Nature, 606(7912):160-164(2022) [pubmed]

submitter keyword: anaerobic,bacteria, organelle, iron

Arash Komeili
contact affiliation	Department of Plant and Microbial Biology, University of California, Berkeley, USA
contact email	komeili@berkeley.edu
lab head
Carly Grant
contact affiliation	University of California, Berkeley
contact email	carly.grant@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD017470

PRIDE project URI

• PRIDE
  1. PXD017470
     1. Label: PRIDE project
     2. Name: Distinct gene clusters drive formation of ferrosome organelles in bacteria