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PXD017470

PXD017470 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDistinct gene clusters drive formation of ferrosome organelles in bacteria
DescriptionCellular iron homeostasis is vital and maintained through tight regulation of iron import, efflux, storage and detoxification1-3. The most common modes of iron storage use proteinaceous compartments, such as ferritins and related proteins4,5. Although lipid-bounded iron compartments have also been described, the basis for their formation and function remains unknown6,7. Here we focus on one such compartment, herein named the 'ferrosome', that was previously observed in the anaerobic bacterium Desulfovibrio magneticus6. Using a proteomic approach, we identify three ferrosome-associated (Fez) proteins that are responsible for forming ferrosomes in D. magneticus. Fez proteins are encoded in a putative operon and include FezB, a P1B-6-ATPase found in phylogenetically and metabolically diverse species of bacteria and archaea. We show that two other bacterial species, Rhodopseudomonas palustris and Shewanella putrefaciens, make ferrosomes through the action of their six-gene fez operon. Additionally, we find that fez operons are sufficient for ferrosome formation in foreign hosts. Using S. putrefaciens as a model, we show that ferrosomes probably have a role in the anaerobic adaptation to iron starvation. Overall, this work establishes ferrosomes as a new class of iron storage organelles and sets the stage for studying their formation and structure in diverse microorganisms.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:37:20.847.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCarly Grant
SpeciesList scientific name: Desulfovibrio magneticus RS-1; NCBI TaxID: 573370; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt G2-S HDMS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-12 03:00:42ID requested
12022-05-31 03:33:10announced
22024-10-22 05:37:21announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41586-022-04741-x;
Grant CR, Amor M, Trujillo HA, Krishnapura S, Iavarone AT, Komeili A, Distinct gene clusters drive formation of ferrosome organelles in bacteria. Nature, 606(7912):160-164(2022) [pubmed]
Keyword List
submitter keyword: anaerobic,bacteria, organelle, iron
Contact List
Arash Komeili
contact affiliationDepartment of Plant and Microbial Biology, University of California, Berkeley, USA
contact emailkomeili@berkeley.edu
lab head
Carly Grant
contact affiliationUniversity of California, Berkeley
contact emailcarly.grant@berkeley.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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