PXD017468

PXD017468 is an original dataset announced via ProteomeXchange.

Title	High Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells
Description	Enteroendocrine cells (EECs) sense intestinal content and release hormones to regulate gastrointestinal activity and systemic metabolism and food intake. Little is known about the molecular make-up of human EEC subtypes and the regulated secretion of individual hormones. Here we describe an organoid-based platform for functional studies of human EECs. EEC formation is induced in vitro by transient expression of NEUROG3. A set of gut organoids was engineered in which the major hormones are fluorescently tagged. A single-cell mRNA atlas was generated for the different EEC subtypes, and their secreted products were recorded by mass-spectrometry. We note key differences with murine EECs, including in hormones, sensory receptors and transcription factors. Notably, several novel hormone-like molecules were identified. Inter-EEC communication is exemplified by Secretin-induced GLP-1 secretion. Indeed, individual EEC subtypes carry receptors for various EEC hormones. This study provides a rich resource to study human EEC development and function.
HostingRepository	PRIDE
AnnounceDate	2020-05-18
AnnouncementXML	Submission_2020-05-18_00:59:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Bauzá Martinez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-12 02:42:41	ID requested
⏵ 1	2020-05-18 00:59:33	announced

Beumer J, Puschhof J, Bauz, á, -Martinez J, Mart, í, nez-Silgado A, Elmentaite R, James KR, Ross A, Hendriks D, Artegiani B, Busslinger GA, Ponsioen B, Andersson-Rolf A, Saftien A, Boot C, Kretzschmar K, Geurts MH, Bar-Ephraim YE, Pleguezuelos-Manzano C, Post Y, Begthel H, van der Linden F, Lopez-Iglesias C, van de Wetering WJ, van der Linden R, Peters PJ, Heck AJR, Goedhart J, Snippert H, Zilbauer M, Teichmann SA, Wu W, Clevers H, High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells. Cell, 181(6):1291-1306.e19(2020) [pubmed]

submitter keyword: Organoids, EEC, Secretome, peptide hormones

Wei Wu
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact email	w.wu1@uu.nl
lab head
Julia Bauzá Martinez
contact affiliation	Utrecht University
contact email	j.bauzamartinez@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017468

PRIDE project URI

• PRIDE
  1. PXD017468
     1. Label: PRIDE project
     2. Name: High Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells