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PXD017458

PXD017458 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhospho-proteomic Profiling Dataset of Chemical Perturbations in Multiple Biological Backgrounds
DescriptionThe Library of Integrated Network-based Cellular Signatures (LINCS) Program aims to create a network-based understanding of biology by cataloging changes in gene expression and other cellular processes that occur when cells are exposed to a variety of perturbing agents. The goal of this project is to test the hypothesis that modulation of phosphorylation-mediated signaling events in response to perturbations can establish new cellular states by altering their epigenetic landscape. We have performed mass spectrometry (MS)-based proteomic assays that target quantitative readouts of phospho-signaling and chromatin modifications in cellular models, following perturbation by compounds with varying mechanisms of action. We have employed 128 compounds and treated 5 cancer and 2 neuronal cell lines. The resulting data collected and tools created have been contributed to the LINCS program for the purpose of making connections among disparate perturbations through phosphoproteomics and chromatin modification signatures in concert with other available data types. The data is publicly available on PanoramaPublic and can be queried using Clue (Broad Institute). Analysis of our data will inform novel therapeutic opportunities and synergies, as dysregulation of phospho-signaling and epigenetic systems are two of the most common molecular etiologies identified in a growing number of diseases.
HostingRepositoryPanoramaPublic
AnnounceDate2022-03-02
AnnouncementXMLSubmission_2022-03-02_15:58:56.836.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKaren Christianson
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Acetyl; Phospho; Oxidation; Label:13C(6)15N(4); Label:13C(6)15N(2); Label:13C(6)
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-11 13:55:12ID requested
12020-08-14 15:51:46announced
22022-03-02 15:58:57announced: Added PubMed Id
Publication List
Dele-Oni DO, Christianson KE, Egri SB, Vaca Jacome AS, DeRuff KC, Mullahoo J, Sharma V, Davison D, Ko T, Bula M, Blanchard J, Young JZ, Litichevskiy L, Lu X, Lam D, Asiedu JK, Toder C, Officer A, Peckner R, MacCoss MJ, Tsai LH, Carr SA, Papanastasiou M, Jaffe JD, Proteomic profiling dataset of chemical perturbations in multiple biological backgrounds. Sci Data, 8(1):226(2021) [pubmed]
Keyword List
submitter keyword: Data-Independent Acquisition, mass spectrometry, proteomics, phospho-signaling, phosphoproteomics
Contact List
Malvina Papanastasiou
contact affiliationBroad Institute of MIT and Harvard
contact emailmalpap@broadinstitute.org
lab head
Karen Christianson
contact affiliationBroad Institute of MIT and Harvard
contact emailkaren@broadinstitute.org
dataset submitter
Full Dataset Link List
Panorama Public dataset URI