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PXD017447

PXD017447 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn situ chromatin interactomics using a chemical bait & trap approach: SILAC datasets
DescriptionElucidating the physiological binding partners of histone post-translational modifications (hPTMs) – i.e. their interactomes – is key to understanding fundamental epigenetic regulatory pathways. Here we use a synthetic biology approach to set a series of hPTM-controlled photo-affinity traps in native chromatin. Using quantitative proteomics, the local interactomes of these chemically customized chromatins are determined. We show that the approach captures transiently interacting factors such methyltransferases and demethylases, as well as previously reported and novel hPTM reader proteins. We also apply this in situ proteomics approach to a recently disclosed cancer-associated histone mutation, H3K4M, revealing a number of perturbed interactions with the mutated tail. Collectively our studies demonstrate that modifying and interrogating native chromatin with chemical precision is a powerful tool for exploring epigenetic regulation and dysregulation at the molecular level.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:32:19.961.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAntony Burton
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C; 6x(13)C; monohydroxylated residue; acetylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-11 08:04:07ID requested
12024-10-07 13:32:25announced
Publication List
Burton AJ, Haugbro M, Gates LA, Bagert JD, Allis CD, Muir TW, In situ chromatin interactomics using a chemical bait and trap approach. Nat Chem, 12(6):520-527(2020) [pubmed]
10.1038/s41557-020-0474-8;
Keyword List
submitter keyword: SILAC
chromatin
histone PTM
Contact List
Tom Muir
contact affiliationPrinceton University
contact emailmuir@princeton.edu
lab head
Antony Burton
contact affiliationPrinceton University
contact emailajburton@princeton.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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