PXD017447

PXD017447 is an original dataset announced via ProteomeXchange.

Title	In situ chromatin interactomics using a chemical bait & trap approach: SILAC datasets
Description	Elucidating the physiological binding partners of histone post-translational modifications (hPTMs) – i.e. their interactomes – is key to understanding fundamental epigenetic regulatory pathways. Here we use a synthetic biology approach to set a series of hPTM-controlled photo-affinity traps in native chromatin. Using quantitative proteomics, the local interactomes of these chemically customized chromatins are determined. We show that the approach captures transiently interacting factors such methyltransferases and demethylases, as well as previously reported and novel hPTM reader proteins. We also apply this in situ proteomics approach to a recently disclosed cancer-associated histone mutation, H3K4M, revealing a number of perturbed interactions with the mutated tail. Collectively our studies demonstrate that modifying and interrogating native chromatin with chemical precision is a powerful tool for exploring epigenetic regulation and dysregulation at the molecular level.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:32:19.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antony Burton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C; 6x(13)C; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-11 08:04:07	ID requested
⏵ 1	2024-10-07 13:32:25	announced

Burton AJ, Haugbro M, Gates LA, Bagert JD, Allis CD, Muir TW, In situ chromatin interactomics using a chemical bait and trap approach. Nat Chem, 12(6):520-527(2020) [pubmed]

10.1038/s41557-020-0474-8;

submitter keyword: SILAC

chromatin

histone PTM

Tom Muir
contact affiliation	Princeton University
contact email	muir@princeton.edu
lab head
Antony Burton
contact affiliation	Princeton University
contact email	ajburton@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD017447

PRIDE project URI

• PRIDE
  1. PXD017447
     1. Label: PRIDE project
     2. Name: In situ chromatin interactomics using a chemical bait & trap approach: SILAC datasets