PXD017441

PXD017441 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass
Description	Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. Root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially expressed proteins (DEPs). From RT1 tissues, 6,156, 7,687 and 7,699 proteins were quantified, and 296, 535 and 384 DEPs were identified in the SDT, D1W and D3W samples, respectively. From RT2 tissues, 7,382, 7,255 and 6,883 proteins were quantified, and 393, 587 and 321 proteins DEPs were identified in the SDT, D1W and D3W samples. Between RT1 and RT2 tissues, very few DEPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DEPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DEPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to choose the proteins (genes) as better defined targets for developing drought tolerant plants.
HostingRepository	PRIDE
AnnounceDate	2020-06-04
AnnouncementXML	Submission_2020-06-04_14:51:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017441
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Howe
SpeciesList	scientific name: Panicum virgatum; NCBI TaxID: 38727;
ModificationList	Methylthio; Deamidated; TMT6plex; Oxidation
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-11 07:39:01	ID requested
⏵ 1	2020-06-04 14:51:02	announced

Dataset with its publication pending

submitter keyword: root-tip zone-specific quantitative proteomics

drought

cell proliferation

cell wall remodeling

hydrophilic proteins

stress proteins

phytohormones.

Ted Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
lab head
Kevin Howe
contact affiliation	USDA-ARS
contact email	kjh46@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017441
     1. Label: PRIDE project
     2. Name: Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass