PXD017432

PXD017432 is an original dataset announced via ProteomeXchange.

Title	Proteolysis of zyxin by HtrA1 induces nuclear translocation of the resulting C-terminal fragment of zyxin
Description	We identified two neo-N termini derived from proteolytic cleavage of the mechanotransducer zyxin at Asp149 and Val276. We correlated these proteolytic events with the activity of caspase-1 and serine protease HtrA1, respectively, and observed nuclear translocation of the fragment zyxin277-572. To evaluate the biological role of nuclear zyxin we used Tandem Mass Tags (TMT)-based quantitative proteomics to compare protein abundances in HeLa cell ectopically expressing zyxin277-572, full length zyxin and control cells.
HostingRepository	PRIDE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-07_06:06:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabio Sabino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; 2-pyrrolidone-5-carboxylic acid (Glu); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-07 06:13:34	ID requested
1	2020-09-07 05:54:07	announced
⏵ 2	2020-09-07 06:06:51	announced	2020-09-07: Updated publication reference for PubMed record(s): 32826880.

Sabino F, Madzharova E, Auf dem Keller U, Cell density-dependent proteolysis by HtrA1 induces translocation of zyxin to the nucleus and increased cell survival. Cell Death Dis, 11(8):674(2020) [pubmed]

submitter keyword: Zyxin, HeLa, TMT, LC-MS/MS

Ulrich auf dem Keller
contact affiliation	Technical University of Denmark
contact email	uadk@dtu.dk
lab head
Fabio Sabino
contact affiliation	Technical University of Denmark
contact email	famisa@dtu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017432
     1. Label: PRIDE project
     2. Name: Proteolysis of zyxin by HtrA1 induces nuclear translocation of the resulting C-terminal fragment of zyxin