PXD017424

PXD017424 is an original dataset announced via ProteomeXchange.

Title	T cells and adipocyte IL-17RC control fat innervation and thermogenesis
Description	The sympathetic nervous system innervates peripheral organs to regulate their function and maintain homeostasis, whereas target cells also produce neurotrophic factors to promote sympathetic innervation1,2. The molecular basis of this bi-directional communication is unknown. Here we use thermogenic adipose tissue from mice as a model system to show that T cells, specifically  T cells, have a crucial role in promoting sympathetic innervation, at least in part by driving the expression of TGF1 in parenchymal cells via the IL-17 receptor complex (IL-17RC). Ablation of IL-17RC specifically in adipose tissue reduces expression of TGF1 in adipocytes, impairs local sympathetic innervation and causes obesity and other metabolic phenotypes that are consistent with defective thermogenesis; innervation can be fully rescued by restoring TGF1 expression. Ablating   cells and the IL-17RC signalling pathway also impairs sympathetic innervation in salivary glands and the lungs. These findings demonstrate coordination between T cells and parenchymal cells to regulate sympathetic innervation.
HostingRepository	PRIDE
AnnounceDate	2020-03-02
AnnouncementXML	Submission_2020-03-02_01:30:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017424
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	mark Jedrychowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-07 00:39:15	ID requested
⏵ 1	2020-03-02 01:30:15	announced

Hu B, Jin C, Zeng X, Resch JM, Jedrychowski MP, Yang Z, Desai BN, Banks AS, Lowell BB, Mathis D, Spiegelman BM, T cells and adipocyte IL-17RC control fat innervation and thermogenesis. Nature, 578(7796):610-614(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Murine Brown Adipose Tissue, TMT based quantitative proteomics

Bruce Spiegelman
contact affiliation	Bruce M. Spiegelman, Ph D. Stanley J. Korsmeyer Professor of Cell Biology and Medicine Dana-Farber Cancer Institute, Harvard Medical School bruce_spiegelman@dfci.harvard.edu 617-632-3567
contact email	Bruce_Spiegelman@dfci.harvard.edu
lab head
mark Jedrychowski
contact affiliation	Harvard Medical School
contact email	mark_jedrychowski@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD017424

PRIDE project URI

• PRIDE
  1. PXD017424
     1. Label: PRIDE project
     2. Name: T cells and adipocyte IL-17RC control fat innervation and thermogenesis