PXD017404

PXD017404 is an original dataset announced via ProteomeXchange.

Title	Complementary peptides (cPEPs) modulate protein translation
Description	MicroRNAs (miRNAs) are small regulatory RNAs that control plant and animal development by regulating post-transcriptional gene expression. Primary transcripts of miRNAs contain small ORFs that can be translated into small peptides (miPEPs). MiPEPs specifically enhance the expression of their cognate miRNA in a positive feedback loop. Here, by addressing the molecular bases of miPEP specificity, we identified an unanticipated mechanism modulating coding gene expression. We found that the miORF itself constitutes a prerequisite for the miPEP to specifically control the expression of its miRNA. We extended this property to any coding gene through the design of artificial peptides. We called these peptides « complementary peptides » (cPEPs) as their external application modulates the translation of their corresponding protein. We discovered here a yet undescribed mechanism of post-transcriptional regulation, opening new promising avenues in fundamental research and applied biology. Quantitative proteomics using LC-MS/MS after co-immunoprecipitation with a HA-tagged cPEP or scrambled peptide was performed to identify interactors of the cPEP or scrambled peptide in co-eluates of plants.
HostingRepository	PRIDE
AnnounceDate	2023-01-09
AnnouncementXML	Submission_2023-01-09_01:05:51.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DelphiVan Haver
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-05 06:10:00	ID requested
⏵ 1	2023-01-09 01:05:52	announced

Dataset with its publication pending

submitter keyword: affinity purification,microRNA, post-transcriptional regulation, complementary peptides

Jean-PhilippeCombier
contact affiliation	Laboratoire de Recherche en Sciences Végétales, CNRS/UPS, 31320 Auzeville-Tolosane, France
contact email	combier@lrsv.ups-tlse.fr
lab head
DelphiVan Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD017404

PRIDE project URI

• PRIDE
  1. PXD017404
     1. Label: PRIDE project
     2. Name: Complementary peptides (cPEPs) modulate protein translation