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PXD017398

PXD017398 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMaTAR25 RNA Antisense Pulldown and Mass Spectrometry
DescriptionIt has been suggested that lncRNAs can interact with transcriptional regulators/co-factors to form ribonucleoprotein (RNP) complexes to regulate the expression of downstream genes in the cell nucleus. To identify potential interacting proteins of MaTAR25, we used two different paired sets of biotin-labeled antisense oligonucleotides targeting MaTAR25 for native RNA antisense oligonucleotide pull-down (RAP) in 4T1 cells followed by qRT-PCR to assess pull-down efficiency. Samples were also eluted from beads for mass spectrometry isobaric tags for relative and absolute quantitative (MS-iTRAQ) analysis to identify proteins that bind to MaTAR25, and PPIB as the corresponding control. We ranked the candidate interactors based on detectable peptides above background in both pair sets of oligonucleotide pull-downs, and selected candidates with at least 2-fold enrichment compared to corresponding PPIB oligo pull-down.
HostingRepositoryPRIDE
AnnounceDate2020-12-23
AnnouncementXMLSubmission_2020-12-23_01:53:19.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKung-Chi Chang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListbiotinylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-05 04:12:53ID requested
12020-12-23 01:53:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mouse, lncRNA, MaTAR25, breast cancer, protein interaction, biotin-oligos
Contact List
David L. Spector
contact affiliationCold Spring Harbor Laboratory
contact emailspector@cshl.edu
lab head
Kung-Chi Chang
contact affiliationCold Spring Harbor Laboratory
contact emailkchang@cshl.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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