PXD017384

PXD017384 is an original dataset announced via ProteomeXchange.

Title	Human GTPBP5 is involved in the late stage of mitoribosome large subunit assembly
Description	Human mitoribosomes are macromolecular complexes essential for translation of 11 mitochondrial mRNAs. The large and the small mitoribosomal subunits undergo a multistep maturation process that requires the involvement of several factors. Among these factors, GTP-binding proteins (GTPBPs) play an important role as GTP hydrolysis can provide energy throughout the assembly stages. In bacteria, many GTPBPs are needed for the maturation of ribosome subunits and, of particular interest for this study, ObgE has been shown to assist in the 50S subunit assembly. Here, we characterize the role of a related human Obg-family member, GTPBP5. We show that GTPBP5 interacts specifically with the large mitoribosomal subunit (mt-LSU) proteins and several late-stage mitoribosome assembly factors, including NSUN4-MTERF4 complex, MRM2 methyltransferase, MALSU1 and MTG1. Interestingly, we find that interaction of GTPBP5 with the mt-LSU is compromised in the presence of a non-hydrolyzable analog of GTP, suggesting a different mechanism of action of this protein in contrast to that of other Obg-family GTPBPs. CRISPR/Cas9-mediated GTPBP5 ablation leads to severe impairment in the oxidative phosphorylation system, concurrent with a decrease in mitochondrial translation, reduced monosome formation and elevated levels of certain mitoribosome assembly factors. Overall, our data indicate an important role of GTPBP5 in mitochondrial function and suggest its involvement in the late-stage maturation of the mt-LSU maturation.
HostingRepository	PRIDE
AnnounceDate	2020-11-09
AnnouncementXML	Submission_2020-12-11_00:46:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-04 08:33:49	ID requested
1	2020-11-09 06:15:31	announced
⏵ 2	2020-12-11 00:46:27	announced	2020-12-11: Updated publication reference for PubMed record(s): 33283228.

Cipullo M, Pearce SF, Lopez Sanchez IG, Gopalakrishna S, Kr, ü, ger A, Schober F, Busch JD, Li X, Wredenberg A, Atanassov I, Rorbach J, Human GTPBP5 is involved in the late stage of mitoribosome large subunit assembly. Nucleic Acids Res, 49(1):354-370(2021) [pubmed]

submitter keyword: mitochondria, LFQ, SILAC

Joanna Rorbach
contact affiliation	Department of Medical Biochemistry and Biophysics, Division of Molecular Metabolism, Karolinska Institutet, Solnavägen 9, 171 65 Solna, Sweden Max Planck Institute Biology of Ageing - Karolinska Institutet Laboratory, Karolinska Institutet, Stockholm, Sweden
contact email	Joanna.rorbach@ki.se
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD017384

PRIDE project URI

• PRIDE
  1. PXD017384
     1. Label: PRIDE project
     2. Name: Human GTPBP5 is involved in the late stage of mitoribosome large subunit assembly