PXD017376

PXD017376 is an original dataset announced via ProteomeXchange.

Title	Identification of cellular proteins binding to the 3’UTR of Programmed Cell Death 4 mRNA
Description	The aim of the project is to identify RNA-binding proteins (RBPs) that interact with the 3’ untranslated region (3’UTR) of the programmed cell death 4 (PDCD4) mRNA. PDCD4 is an inflammatory tumor suppressor gene. The translation of PDCD4 mRNA is regulated by multiple RBPs. The aim is to identify RBPs which are critical for regulating PDCD4 mRNA translation by binding to its 3’UTR. For this purpose, a biotinylated PDCD4 3’UTR RNA was incubated with cytoplasmic lysate from MCF7 human breast carcinoma cells and the RNA-protein complexes pulled down by streptavidin affinity chromatography. The proteins were then eluted and resolved by SDS-PAGE. One particular protein band, migrating close to 55 KDa marker was cut out and submitted for proteomic analysis by in gel digestion and mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2020-12-11
AnnouncementXML	Submission_2020-12-11_00:41:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chhaya Patole
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-04 07:28:28	ID requested
⏵ 1	2020-12-11 00:41:55	announced

Kumar R, Poria DK, Ray PS, RNA-binding proteins La and HuR cooperatively modulate translation repression of PDCD4 mRNA. J Biol Chem, 296():100154(2021) [pubmed]

submitter keyword: RNA-protein interaction, RNA-binding protein, 3’ untranslated region, programmed cell death 4, Mass Spectrometry

Professor Partho Sarothi Ray
contact affiliation	IISER Kolkata Department of Biological Sciences Mohanpur, Dist. Nadia West Bengal, India
contact email	psray@iiserkol.ac.in
lab head
Chhaya Patole
contact affiliation	Instem
contact email	cpatole@instem.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD017376

PRIDE project URI

• PRIDE
  1. PXD017376
     1. Label: PRIDE project
     2. Name: Identification of cellular proteins binding to the 3’UTR of Programmed Cell Death 4 mRNA