PXD017375

PXD017375 is an original dataset announced via ProteomeXchange.

Title	Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts treatment on HeLa cells
Description	Nothapodytes nimmoniana is a natural source of camptothecin, a known anticancer drug. Stem and leaf extracts of Nothapodytes nimmoniana were studied to assess its in-vitro action on cancer progression events in a cervical cancer cell line, the HeLa. As a well-studied ideal model system, the HeLa has been chosen and the effects of extracts on cancer progression events have been traced out. HeLa cells and media samples were analyzed for differentially expressed proteins and metabolites respectively on RRLC-ESI-QTOFMS. The CBFA2T1, cysteine-rich protein 2-binding protein, Zinc finger protein 788, transcription factor RFX3 and angiomotin-like protein 1 were significantly expressed proteins while 3-Hydroxysuberic acid, Indole-3-carboxylic acid, N8-Acetylspermidine, L-Octanoylcarnitine were significantly expressed metabolites. Aminoacyl-tRNA biosynthesis, purine metabolism, and valine, leucine, arginine, proline metabolism were the most prominent pathways. The multi-omics approach has the greatest metabolites and proteins profiling potential hence was applied here to find signatures of N. nimmoniana extracts treatment of cervical cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:40.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amey Shirolkar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-04 07:16:13	ID requested
1	2024-10-08 11:39:34	announced
⏵ 2	2024-10-22 05:01:41	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Nothapodytes nimmoniana (J. Graham), anticancer, proteomics, metabolomics, RRLC-ESI-QTOFMS, HeLa, camptothecin

Sharad Daulatrao Pawar
contact affiliation	Research Officer, Department of Pharmacology, National Research Institute of Basic Ayurvedic Sciences (NRIBAS), Kothrud, Pune - 411038, Maharashtra, India
contact email	sd_pawar@yahoo.com
lab head
Amey Shirolkar
contact affiliation	Laboratory Technician, RAIFR, Pune
contact email	amey_593@rediffmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017375

PRIDE project URI

• PRIDE
  1. PXD017375
     1. Label: PRIDE project
     2. Name: Proteomic and metabolomic analysis of Nothapodytes nimmoniana (J. Graham) extracts treatment on HeLa cells