PXD017372

PXD017372 is an original dataset announced via ProteomeXchange.

Title	Secretome Comparison of Mesenchymal Stromal Cells and Immature Osteoblasts
Description	Osteoblasts are a key component of the endosteal hematopoietic stem cell (HSC) niche and have long been recognized with strong hematopoietic supporting activity. Osteoblast conditioned media (OCM) enhances the growth of hematopoietic progenitors in culture and modulate their engraftment activity. We aimed to characterize the hematopoietic supporting activity of OCM by comparing the secretome of immature osteoblasts to that of their precursor, mesenchymal stromal cells (MSC). Over 300 secreted proteins were quantified by mass spectroscopy in media conditioned with MSC or osteoblasts, with 47 being differentially expressed.
HostingRepository	PRIDE
AnnounceDate	2020-07-17
AnnouncementXML	Submission_2020-07-21_18:19:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Owen HOvey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-04 02:51:24	ID requested
1	2020-07-16 17:56:55	announced
⏵ 2	2020-07-21 18:19:55	announced	2020-07-22: Updated publication reference for PubMed record(s): 32666692.

Hovey O, Pasha R, Lehmann Z, Pineault N, Insights into the Hematopoietic Regulatory Activities of Osteoblast by Secretomics. Proteomics, ():e2000036(2020) [pubmed]

submitter keyword: Mesenchymal Stromal Cells, Osteoblasts, Secretome

Nicolas Pineault
contact affiliation	Canadian Blood Services, Centre for Innovation, Ottawa, Ontario, Canada; Biochemistry, Microbiology and Immunology department, University of Ottawa, Ottawa, Canada.
contact email	nicolas.pineault@blood.ca
lab head
Owen HOvey
contact affiliation	University of Western Ontario
contact email	ohovey@uwo.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017372

PRIDE project URI

• PRIDE
  1. PXD017372
     1. Label: PRIDE project
     2. Name: Secretome Comparison of Mesenchymal Stromal Cells and Immature Osteoblasts