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PXD017351

PXD017351 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSsrp1 co-immunoprecipitation and mass spectrometry
DescriptionEndogenous retroviruses (ERVs) were usually silenced by various histone modifications on histone H3 variants and respective histone chaperones in embryonic stem cells (ESCs). However, it is still unknown whether chaperones of other histones could repress ERVs. Here, we show that H2A/H2B histone chaperone FACT plays a critical role in silencing ERVs and ERV-derived cryptic promoters in ESCs. Loss of FACT component Ssrp1 activated MERVL whereas the re-introduction of Ssrp1 rescued the phenotype. Additionally, Ssrp1 interacted with MERVL and suppressed cryptic transcription of MERVL-fused genes. Remarkably, Ssrp1 interacted with and recruited H2B deubiquitinase Usp7 to Ssrp1 target genes. Suppression of Usp7 caused similar phenotypes as loss of Ssrp1. Furthermore, Usp7 acted by deubiquitinating H2Bub and thereby repressed the expression of MERVL-fused genes. Taken together, our study uncovers a unique mechanism by which FACT complex silences ERVs and ERV-derived cryptic promoters in ESCs.
HostingRepositoryPRIDE
AnnounceDate2020-08-24
AnnouncementXMLSubmission_2020-09-13_22:26:02.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWeiyu Zhang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-31 05:55:35ID requested
12020-08-24 02:26:58announced
22020-09-13 22:26:03announced2020-09-14: Updated publication reference for PubMed record(s): 32894293.
Publication List
Chen F, Zhang W, Xie D, Gao T, Dong Z, Lu X, Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters. Nucleic Acids Res, 48(18):10211-10225(2020) [pubmed]
Keyword List
submitter keyword: Ssrp1, co-immunoprecipitation, mass spectrometry, ESC
Contact List
Xinyi Lu
contact affiliationState Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, People's Republic of China; College of Pharmacy, Nankai University, Tianjin, People's Republic of China.
contact emailluxy@nankai.edu.cn
lab head
Weiyu Zhang
contact affiliationNankai University
contact emailch8316f5eyu@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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