PXD017351

PXD017351 is an original dataset announced via ProteomeXchange.

Title	Ssrp1 co-immunoprecipitation and mass spectrometry
Description	Endogenous retroviruses (ERVs) were usually silenced by various histone modifications on histone H3 variants and respective histone chaperones in embryonic stem cells (ESCs). However, it is still unknown whether chaperones of other histones could repress ERVs. Here, we show that H2A/H2B histone chaperone FACT plays a critical role in silencing ERVs and ERV-derived cryptic promoters in ESCs. Loss of FACT component Ssrp1 activated MERVL whereas the re-introduction of Ssrp1 rescued the phenotype. Additionally, Ssrp1 interacted with MERVL and suppressed cryptic transcription of MERVL-fused genes. Remarkably, Ssrp1 interacted with and recruited H2B deubiquitinase Usp7 to Ssrp1 target genes. Suppression of Usp7 caused similar phenotypes as loss of Ssrp1. Furthermore, Usp7 acted by deubiquitinating H2Bub and thereby repressed the expression of MERVL-fused genes. Taken together, our study uncovers a unique mechanism by which FACT complex silences ERVs and ERV-derived cryptic promoters in ESCs.
HostingRepository	PRIDE
AnnounceDate	2020-08-24
AnnouncementXML	Submission_2020-09-13_22:26:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weiyu Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-31 05:55:35	ID requested
1	2020-08-24 02:26:58	announced
⏵ 2	2020-09-13 22:26:03	announced	2020-09-14: Updated publication reference for PubMed record(s): 32894293.

Chen F, Zhang W, Xie D, Gao T, Dong Z, Lu X, Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters. Nucleic Acids Res, 48(18):10211-10225(2020) [pubmed]

submitter keyword: Ssrp1, co-immunoprecipitation, mass spectrometry, ESC

Xinyi Lu
contact affiliation	State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, People's Republic of China; College of Pharmacy, Nankai University, Tianjin, People's Republic of China.
contact email	luxy@nankai.edu.cn
lab head
Weiyu Zhang
contact affiliation	Nankai University
contact email	ch8316f5eyu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017351

PRIDE project URI

• PRIDE
  1. PXD017351
     1. Label: PRIDE project
     2. Name: Ssrp1 co-immunoprecipitation and mass spectrometry