PXD017349

PXD017349 is an original dataset announced via ProteomeXchange.

Title	Iron deficiency response of Corynebacterium glutamicum
Description	The response to iron limitation of the Gram-positive soil bacterium Corynebacterium glutamicum was analyzed with respect to proteome during growth in glucose minimal medium. C. glutamicum cells were grown at regular (36 µM) and low (1 µM) iron concentrations and harvested in the late exponential growth phase. Between 1056 and 1357 proteins were detected (1% false discovery rate, FDR) in the measurements of three biological replicates including technical replicates each for low and high iron conditions with a proteomic shotgun analysis using nanoLC-MS/MS, covering in total 1555 proteins. By SWATH-MS measurements, relative levels of 1123 proteins could be quantified. Thereof, 66 proteins exhibited at least two-fold altered ratios with a p-value ≤0.05 in iron-deprived cells compared to the cells cultivated with 36 μM iron. 38 proteins showed a ≥2-fold higher level under iron limitation, 16 of which were members of the DtxR regulon. The levels of 28 proteins were at least 2-fold lower under iron limitation, 10 of which were members of the RipA regulon.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:10.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tino Polen
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-31 02:05:24	ID requested
1	2020-04-14 23:12:49	announced
⏵ 2	2024-10-22 05:02:18	announced	2024-10-22: Updated project metadata.

K, ü, berl A, Mengus-Kaya A, Polen T, Bott M, The Iron Deficiency Response of Corynebacterium glutamicum and a Link to Thiamine Biosynthesis. Appl Environ Microbiol, 86(10):(2020) [pubmed]

10.1128/aem.00065-20;

curator keyword: Biological

submitter keyword: Corynebacterium glutamicum,iron

Tino Polen
contact affiliation	IBG-1: Biotechnology Forschungszentrum Juelich
contact email	t.polen@fz-juelich.de
lab head
Tino Polen
contact affiliation	Forschungszentrum Jülich
contact email	t.polen@fz-juelich.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017349

PRIDE project URI

• PRIDE
  1. PXD017349
     1. Label: PRIDE project
     2. Name: Iron deficiency response of Corynebacterium glutamicum