PXD017341

PXD017341 is an original dataset announced via ProteomeXchange.

Title	Titin BioID - protein and peptide pull-down
Description	Proximity proteomics has greatly advanced the analysis of native protein complexes and subcellular structures in culture, but has not been amenable to study development and disease in vivo. Here, we generated a knock-in mouse with the biotin ligase (BioID) inserted at titin’s Z-disc region to identify protein networks that connect the sarcomere to signal transduction and metabolism. Our census of the sarcomeric proteome from neonatal to adult heart and quadriceps reveals how perinatal signalling, protein homeostasis and the shift to adult energy metabolism shape the properties of striated muscle cells. Mapping biotinylation sites to sarcomere structures refined our understanding of myofilament dynamics and supports the hypothesis that myosin filaments penetrate Z-discs to dampen contraction. Extending this proof of concept study to BioID fusion proteins generated with Crispr/CAS9 in animal models recapitulating human pathology will facilitate the future analysis of molecular machines and signalling hubs in physiological, pharmacological, and disease context.
HostingRepository	PRIDE
AnnounceDate	2020-05-18
AnnouncementXML	Submission_2020-07-01_22:38:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marieluise Kirchner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-31 01:09:13	ID requested
1	2020-05-18 02:40:03	announced
⏵ 2	2020-07-01 22:38:09	announced	2020-07-02: Updated publication reference for PubMed record(s): 32561764.

Rudolph F, Fink C, H, ü, ttemeister J, Kirchner M, Radke MH, Lopez Carballo J, Wagner E, Kohl T, Lehnart SE, Mertins P, Gotthardt M, Deconstructing sarcomeric structure-function relations in titin-BioID knock-in mice. Nat Commun, 11(1):3133(2020) [pubmed]

curator keyword: Technical

submitter keyword: mouse, BioID, titin, biotinylation sites

Michael Gotthardt
contact affiliation	Neuromuscular and Cardiovascular Cell Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, 13125 Berlin, Germany
contact email	gotthardt@mdc-berlin.de
lab head
Marieluise Kirchner
contact affiliation	Max Delbrueck Center, Berlin
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017341

PRIDE project URI

• PRIDE
  1. PXD017341
     1. Label: PRIDE project
     2. Name: Titin BioID - protein and peptide pull-down