PXD017340

PXD017340 is an original dataset announced via ProteomeXchange.

Title	Serum-based proteomics reveals lipid metabolic and immunoregulatory 、dysregulation in cervical artery dissection with stroke
Description	Cervical artery dissection (CAD) is an important causal factor for stroke in young and middle-aged individuals and presents a great burden to the individual stroke victim. However, the pathophysiological mechanisms underlying CAD remain unknown. Here, an isobaric tagging for relative and absolute quantitation (iTRAQ)-based quantitative proteomic approach was performed, to identify differentially expressed proteins in serum samples obtained from spontaneous CAD and non-CAD ischemic stroke subjects. Differential protein expression was analyzed for Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway over-representation, and six differential proteins were selected for enzyme-linked immunosorbent assay (ELISA) validation. Through KEGG analysis, the significantly differentiated proteins were primarily involved in immunoregulation, blood coagulation, and lipid metabolism. For the first time, differential expressions of apolipoprotein B (APOB), apolipoprotein C-I (APOC-I), lipopolysaccharide-binding protein (LBP), vascular cell adhesion molecule 1(VCAM1), fibulin-1 (FBLN1), and ficolin-2 (FCN2) were confirmed as being significantly upregulated in CAD as compared to non-CAD ischemic stroke subjects. In conclusion, proteomic analysis reveals that early perturbation of immunoregulation and lipid metabolism may be involved in the pathophysiology of CAD. Specifically, the panel of six proteins identified are promising as serum-based predictors for the detection of increased CAD risk in stroke subjects.
HostingRepository	iProX
AnnounceDate	2020-01-30
AnnouncementXML	Submission_2023-08-27_21:00:50.136.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongtao Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-30 19:00:56	ID requested
⏵ 1	2023-08-27 21:00:51	announced

Yang Y, Peng J, Wang S, Huang J, Ran H, Chen K, Zhou Z, Serum-Based Proteomics Reveals Lipid Metabolic and Immunoregulatory Dysregulation in Cervical Artery Dissection With Stroke. Front Neurol, 11():352(2020) [pubmed]

submitter keyword: Cervical artery dissection, Stroke, Serum, Immunoregulation, Lipid metabolism

Zhenhua Zhou
contact affiliation	Department of Neurology, Chongqing Renji Hospital, University of Chinese Academy of Sciences
contact email	exploiter001@126.com
lab head
Yongtao Yang
contact affiliation	Department of Neurology, Chongqing Renji Hospital, University of Chinese Academy of Sciences
contact email	smile_z@163.com
dataset submitter

iProX dataset URI