PXD017337

PXD017337 is an original dataset announced via ProteomeXchange.

Title	Identification of PRDM9 partners
Description	PRDM9 specifies the sites of meiotic DNA double strand break that initiate meiotic recombination in mice and humans. PRDM9 is known to bind to specific DNA sequences with its DNA binding domain, to induce H3K4me3 and H3K36me3 to adjacent nucleosomes through its methyltransferase activity, and to recruit or activate the meiotic DSB machinery. To understand how PRDM9 executes these various steps, we set up to identify its partners. This was performed by a proteomic approach where protein extracts from mouse testis were immunoprecipitated with anti-PRDM9 antibody for mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:09.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oana Vigy
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-30 07:31:04	ID requested
1	2020-12-02 23:59:31	announced
⏵ 2	2024-10-22 05:16:10	announced	2024-10-22: Updated project metadata.

Imai Y, Biot M, Cl, é, ment JA, Teragaki M, Urbach S, Robert T, Baudat F, Grey C, de Massy B, PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment. Elife, 9():(2020) [pubmed]

10.7554/elife.57117;

submitter keyword: DNA Breaks,Meiosis, Recombination, Chromatin

Bernard de Massy
contact affiliation	Institute of Human Genetics, Centre National de la Recherche Scientifique, Univ Montpellier, Montpellier, France
contact email	Bernard.de-massy@igh.cnrs.fr
lab head
Oana Vigy
contact affiliation	FPP CNRS
contact email	oana.vigy@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD017337

PRIDE project URI

• PRIDE
  1. PXD017337
     1. Label: PRIDE project
     2. Name: Identification of PRDM9 partners