PXD017335

PXD017335 is an original dataset announced via ProteomeXchange.

Title	A dedicated ubiquitin-K48/63R variant-based affinity approach specifically identifies both high-risk and low-risk substrates of the HPV E6-E6AP ubiquitin ligase complex
Description	To identify potential substrate proteins of both low risk and high risk E6 proteins, we established an affinity-based enrichment approach using a dedicated biotin-tagged ubiquitin variant that in the absence of the E6 proteins, cannot or only poorly be used by E6AP for ubiquitination. Enriched proteins were identified by high-resolution mass spectrometry (LC-MS/MS) and label-free quantification. E6-mediated ubiquitination/degradation of some of the proteins identified were validated in vitro and within cells by using recombinant proteins and in transient transfection experiments in cells demonstrating the potential of this approach.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:33.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-30 06:20:26	ID requested
1	2020-08-30 22:48:38	announced
⏵ 2	2024-10-22 05:11:34	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: E6, UBE3A,E6-E6AP ubiquitin ligase, HPV-16, Human papillomavirus (HPV), HPV-11

Florian Stengel
contact affiliation	Department of Biology, University of Konstanz, Germany
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017335

PRIDE project URI

• PRIDE
  1. PXD017335
     1. Label: PRIDE project
     2. Name: A dedicated ubiquitin-K48/63R variant-based affinity approach specifically identifies both high-risk and low-risk substrates of the HPV E6-E6AP ubiquitin ligase complex