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PXD017335

PXD017335 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA dedicated ubiquitin-K48/63R variant-based affinity approach specifically identifies both high-risk and low-risk substrates of the HPV E6-E6AP ubiquitin ligase complex
DescriptionTo identify potential substrate proteins of both low risk and high risk E6 proteins, we established an affinity-based enrichment approach using a dedicated biotin-tagged ubiquitin variant that in the absence of the E6 proteins, cannot or only poorly be used by E6AP for ubiquitination. Enriched proteins were identified by high-resolution mass spectrometry (LC-MS/MS) and label-free quantification. E6-mediated ubiquitination/degradation of some of the proteins identified were validated in vitro and within cells by using recombinant proteins and in transient transfection experiments in cells demonstrating the potential of this approach.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:11:33.683.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian Stengel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-30 06:20:26ID requested
12020-08-30 22:48:38announced
22024-10-22 05:11:34announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: E6, UBE3A,E6-E6AP ubiquitin ligase, HPV-16, Human papillomavirus (HPV), HPV-11
Contact List
Florian Stengel
contact affiliationDepartment of Biology, University of Konstanz, Germany
contact emailflorian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliationUniversität Konstanz
contact emailflorian.stengel@uni-konstanz.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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