PXD017335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A dedicated ubiquitin-K48/63R variant-based affinity approach specifically identifies both high-risk and low-risk substrates of the HPV E6-E6AP ubiquitin ligase complex |
Description | To identify potential substrate proteins of both low risk and high risk E6 proteins, we established an affinity-based enrichment approach using a dedicated biotin-tagged ubiquitin variant that in the absence of the E6 proteins, cannot or only poorly be used by E6AP for ubiquitination. Enriched proteins were identified by high-resolution mass spectrometry (LC-MS/MS) and label-free quantification. E6-mediated ubiquitination/degradation of some of the proteins identified were validated in vitro and within cells by using recombinant proteins and in transient transfection experiments in cells demonstrating the potential of this approach. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:11:33.683.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Stengel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitination signature dipeptidyl lysine |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-30 06:20:26 | ID requested | |
1 | 2020-08-30 22:48:38 | announced | |
⏵ 2 | 2024-10-22 05:11:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: E6, UBE3A,E6-E6AP ubiquitin ligase, HPV-16, Human papillomavirus (HPV), HPV-11 |
Contact List
Florian Stengel |
contact affiliation | Department of Biology, University of Konstanz, Germany |
contact email | florian.stengel@uni-konstanz.de |
lab head | |
Florian Stengel |
contact affiliation | Universität Konstanz |
contact email | florian.stengel@uni-konstanz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017335 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD017335
- Label: PRIDE project
- Name: A dedicated ubiquitin-K48/63R variant-based affinity approach specifically identifies both high-risk and low-risk substrates of the HPV E6-E6AP ubiquitin ligase complex