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PXD017329

PXD017329 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGFP-CSB and GFP-UVSSA Domain-specific interactors
DescriptionThe response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA repair complex, including transcription factor IIH (TFIIH), is recruited are largely unknown. Here, we define the assembly mechanism of the TCR complex in human isogenic knockout cells. We show that TCR is initiated by RNAPIIobound CSB, which recruits CSA through a newly identified CSA-interaction motif (CIM). Once recruited, CSA facilitates the association of UVSSA with stalled RNAPIIo. Importantly, we find that UVSSA is the key factor that recruits the TFIIH complex in a manner that is stimulated by CSB and CSA. Together these findings reveal a sequential and highly cooperative assembly mechanism of TCR proteins and reveal the mechanism for TFIIH recruitment to DNA damage-stalled RNAPIIo to initiate repair.
HostingRepositoryPRIDE
AnnounceDate2020-04-14
AnnouncementXMLSubmission_2020-05-05_22:33:54.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRomán González-Prieto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-30 00:19:53ID requested
12020-04-13 23:00:47announced
22020-05-05 22:33:56announced2020-05-06: Updated publication reference for PubMed record(s): 32355176.
Publication List
van der Weegen Y, Golan-Berman H, Mevissen TET, Apelt K, Gonz, á, lez-Prieto R, Goedhart J, Heilbrun EE, Vertegaal ACO, van den Heuvel D, Walter JC, Adar S, Luijsterburg MS, The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Nat Commun, 11(1):2104(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: CSB, UVSSA, TC-NER
Contact List
Alfred C.O. Vertegaal
contact affiliationDepartment of Cell and Chemical Biology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands
contact emailvertegaal@lumc.nl
lab head
Román González-Prieto
contact affiliationLeiden University Medical Center (LUMC)
contact emailR.Gonzalez_Prieto@lumc.nl
dataset submitter
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Dataset FTP location
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