PXD017328

PXD017328 is an original dataset announced via ProteomeXchange.

Title	Stress enhances secretory autophagy increasing extracellular Bdnf maturation
Description	Stress response is a fundamental mechanism to maintain a healthy homeostasis and its disruption is implicated in several psychiatric disorders. Autophagy is one of the mechanisms regulating homeostasis, and it is known to be activated by cellular stressors such as starvation. In a previous study, we revealed that glucocorticoid (GC)-mediated stress also activates macroautophagy via the stress-responsive co-chaperone FK506-binding protein 51 (FKBP51). In this study we reveal that GC-mediated stress enhances secretory autophagy, a recently described Atg-dependent secretory pathway, via FKBP51. Strikingly we detected the matrix metallopeptidase 9 (Mmp9) as one of the stress-induced secreted proteins. Furthermore we found that enhanced Mmp9 secretion increases both in vitro and in vivo the cleavage of pro-brain derived neurotrophic factor (proBdnf) to its mature form (mBdnf), a key protein in neuroplasticity. This unravelled mechanism can represent a link between stress and the development of psychiatric disorders.
HostingRepository	PRIDE
AnnounceDate	2021-08-26
AnnouncementXML	Submission_2021-08-26_06:46:03.024.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017328
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Giuseppina Maccarrone
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-29 23:58:23	ID requested
⏵ 1	2021-08-26 06:46:03	announced

Martinelli S, Anderzhanova EA, Bajaj T, Wiechmann S, Dethloff F, Weckmann K, Heinz DE, Ebert T, Hartmann J, Geiger TM, D, ö, ngi M, Hafner K, P, ö, hlmann ML, Jollans L, Philipsen A, Schmidt SV, Schmidt U, Maccarrone G, Stein V, Hausch F, Turck CW, Schmidt MV, Gellner AK, Kuster B, Gassen NC, Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion. Nat Commun, 12(1):4643(2021) [pubmed]

submitter keyword: Human, LC-MSMS, interactome

Maccarrone Giuseppina
contact affiliation	Department of Translational Research in Psychiatry, Proteomics Core Unit, Max Planck Institute of Psychiatry, Germany
contact email	maccarrone@psych.mpg.de
lab head
Giuseppina Maccarrone
contact affiliation	Max Planck Institute of Psychiatry
contact email	maccarrone@psych.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017328
     1. Label: PRIDE project
     2. Name: Stress enhances secretory autophagy increasing extracellular Bdnf maturation