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PXD017322

PXD017322 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMaximizing Confidence and Sensitivity in quantitative Phosphoproteomics
DescriptionProteome-wide analysis of phosphorylation events is a challenging, yet essential, task for the comprehensive and unbiased investigation of kinase action. Here we developed a phosphoproteomic approach in which quantitation consistency among reversed isotopically labeled samples is used as a central filtering rule for achieving reliability with minimal loss of data content. Exclusion of non-reverting data-points from the dataset not only reduces quantitation error and variation, but also reduces false positive identifications. This dataset corresponds to SILAC labeling swapping control experiments.
HostingRepositoryMassIVE
AnnounceDate2021-01-25
AnnouncementXMLSubmission_2021-01-25_06:32:33.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVitor Faca
SpeciesList scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationListPhospho; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-29 06:47:28ID requested
12021-01-25 06:32:33announced
Publication List
Faca VM, Sanford EJ, Tieu J, Comstock W, Gupta S, Marshall S, Yu H, Smolka MB, Maximized quantitative phosphoproteomics allows high confidence dissection of the DNA damage signaling network. Sci Rep, 10(1):18056(2020) [pubmed]
Keyword List
submitter keyword: Quantitative Phosphoproteomics, Kinase Targets
Contact List
Marcus Smolka
contact affiliationCornell University
contact emailmbs266@cornell.edu
lab head
Vitor Faca
contact affiliationUniversity of Sao Paulo
contact emailvitor.faca@fmrp.usp.br
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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