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PXD017313

PXD017313 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine
DescriptionThe skin plays a crucial role in host defences against microbial attack and the innate cells must provide the immune system with sufficient information to organize these defences. This unique feature makes the skin a promising site for vaccine administration. Although cellular innate immune events during vaccination have been widely studied, initial events remain poorly understood. Our aim is to determine molecular biomarkers of skin innate reaction after intradermal (i.d.) immunization. Using an ex vivo human explant model from healthy donors, we investigated by NanoLC-MS/MS analysis and MALDI-MSI imaging, to detect innate molecular events (lipids, metabolites, proteins) few hours after i.d. administration of seasonal trivalent influenza vaccine (TIV). This multimodel approach allowed to identify early molecules differentially expressed in dermal and epidermal layers at 4 and 18 h after TIV immunization compared with control PBS. In the dermis, the most relevant network of proteins upregulated were related to cell-to-cell signaling and cell trafficking. The molecular signatures detected were associated with chemokines such as CXCL8, a chemoattractant of neutrophils. In the epidermis, the most relevant networks were associated with activation of antigen-presenting cells and related to CXCL10. Our study proposes a novel step-forward approach to identify biomarkers of skin innate reaction.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:16:20.055.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaxence Wisztorski
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-29 03:48:40ID requested
12020-01-29 23:58:49announced
22024-10-22 04:16:24announced2024-10-22: Updated project metadata.
Publication List
Gonnet J, Poncelet L, Meriaux C, Gon, ç, alves E, Weiss L, Tchitchek N, Pedruzzi E, Soria A, Boccara D, Vogt A, Bonduelle O, Hamm G, Ait-Belkacem R, Stauber J, Fournier I, Wisztorski M, Combadiere B, Mechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine. J Proteomics, 216():103670(2020) [pubmed]
10.1016/j.jprot.2020.103670;
Keyword List
submitter keyword: skin
biomarkers
Mass Spectrometry
intradermal vaccination
innate immunity
Contact List
Michel Salzet
contact affiliationUniversité de Lille - INSERM U1192 - Laboratoire PRISM
contact emailmichel.salzet@univ-lille.fr
lab head
Maxence Wisztorski
contact affiliationUniversity of Lille
contact emailmaxence.wisztorski@univ-lille1.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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