PXD017313

PXD017313 is an original dataset announced via ProteomeXchange.

Title	Mechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine
Description	The skin plays a crucial role in host defences against microbial attack and the innate cells must provide the immune system with sufficient information to organize these defences. This unique feature makes the skin a promising site for vaccine administration. Although cellular innate immune events during vaccination have been widely studied, initial events remain poorly understood. Our aim is to determine molecular biomarkers of skin innate reaction after intradermal (i.d.) immunization. Using an ex vivo human explant model from healthy donors, we investigated by NanoLC-MS/MS analysis and MALDI-MSI imaging, to detect innate molecular events (lipids, metabolites, proteins) few hours after i.d. administration of seasonal trivalent influenza vaccine (TIV). This multimodel approach allowed to identify early molecules differentially expressed in dermal and epidermal layers at 4 and 18 h after TIV immunization compared with control PBS. In the dermis, the most relevant network of proteins upregulated were related to cell-to-cell signaling and cell trafficking. The molecular signatures detected were associated with chemokines such as CXCL8, a chemoattractant of neutrophils. In the epidermis, the most relevant networks were associated with activation of antigen-presenting cells and related to CXCL10. Our study proposes a novel step-forward approach to identify biomarkers of skin innate reaction.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:16:20.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maxence Wisztorski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-29 03:48:40	ID requested
1	2020-01-29 23:58:49	announced
⏵ 2	2024-10-22 04:16:24	announced	2024-10-22: Updated project metadata.

Gonnet J, Poncelet L, Meriaux C, Gon, ç, alves E, Weiss L, Tchitchek N, Pedruzzi E, Soria A, Boccara D, Vogt A, Bonduelle O, Hamm G, Ait-Belkacem R, Stauber J, Fournier I, Wisztorski M, Combadiere B, Mechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine. J Proteomics, 216():103670(2020) [pubmed]

10.1016/j.jprot.2020.103670;

submitter keyword: skin

biomarkers

Mass Spectrometry

intradermal vaccination

innate immunity

Michel Salzet
contact affiliation	Université de Lille - INSERM U1192 - Laboratoire PRISM
contact email	michel.salzet@univ-lille.fr
lab head
Maxence Wisztorski
contact affiliation	University of Lille
contact email	maxence.wisztorski@univ-lille1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017313

PRIDE project URI

• PRIDE
  1. PXD017313
     1. Label: PRIDE project
     2. Name: Mechanisms of innate events during skin reaction following intradermal injection of seasonal influenza vaccine