PXD017309

PXD017309 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics to identify nuclear RNA-binding proteins of Malat1
Description	The long non-coding RNA Malat1 has been implicated in several human cancers, while the mechanism of action is not completely understood. As RNAs in cells function in the context of RBPs identification of their RNA-binding proteins can shed light on their functionality. We here performed quantitative interactomics of 14 non-overlapping fragments covering the full length of Malat1 to identify possible nuclear interacting proteins. Overall, we identified 35 candidates including 14 already known binders, which are able to interact with Malat1 in the nucleus. Furthermore, the use of fragments along the full-length RNA allowed us to reveal two hotspots for protein binding, one in the 5???-region and one in the 3???-region of Malat1. Our results provide confirmation on previous RNA-protein interaction studies and suggest new candidates for functional investigations.
HostingRepository	PRIDE
AnnounceDate	2020-03-30
AnnouncementXML	Submission_2020-03-30_05:56:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-29 00:08:13	ID requested
1	2020-01-29 06:01:04	announced
2	2020-02-18 07:27:13	announced	2020-02-18: Updated publication reference for PubMed record(s): 32050583.
⏵ 3	2020-03-30 05:56:10	announced	2020-03-30: Updated project metadata.

Scherer M, Levin M, Butter F, Scheibe M, Quantitative Proteomics to Identify Nuclear RNA-Binding Proteins of Malat1. Int J Mol Sci, 21(3):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Malat1

lncRNA

RBPs

interactome

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB) Ackermannweg 4 55128 Mainz
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017309

PRIDE project URI

• PRIDE
  1. PXD017309
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics to identify nuclear RNA-binding proteins of Malat1