PXD017308

PXD017308 is an original dataset announced via ProteomeXchange.

Title	A divergent cyclin/cyclin-dependent kinase complex controls progression through the atypical replicative cycles during Plasmodium berghei gametogony
Description	Cell-cycle transitions are generally triggered by variations in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have evolved unique cell-cycles with a repertoire of ancestral CDKs and cyclins whose functions and interdependency remain elusive. Here, we show that the divergent Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical cell-cycle regulator of gametogony required for transmission to the mosquito. It phosphorylates canonical CDK motifs on components of the pre-replicative complex and is essential for DNA replication. We also provide evidence for indirect regulation of the concomitant progression through M-phase. Over a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2) with no evidence of SOC2 cycling through transcription, translation nor degradation. Our results present evidence that during Plasmodium gametogony, a unique and divergent cyclin/CDK pair evolved to fulfil the functional space of multiple eukaryotic cell-cycle kinases controlling S-phase entry and progression through M-phase.
HostingRepository	PRIDE
AnnounceDate	2020-02-25
AnnouncementXML	Submission_2020-07-08_06:33:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017308
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	alexandre Hainard
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	Phospho; Deamidated; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-28 07:16:43	ID requested
1	2020-02-25 08:19:08	announced
⏵ 2	2020-07-08 06:33:17	announced	2020-07-08: Updated publication reference for PubMed record(s): 32568069.

Balestra AC, Zeeshan M, Rea E, Pasquarello C, Brusini L, Mourier T, Subudhi AK, Klages N, Arboit P, Pandey R, Brady D, Vaughan S, Holder AA, Pain A, Ferguson DJ, Hainard A, Tewari R, Brochet M, A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission. Elife, 9():(2020) [pubmed]

submitter keyword: plasmodium berghei, CRK5, SOC2, Co-IP

Mathieu Brochet
contact affiliation	University of Geneva, Faculty of Medicine, Geneva, Switzerland
contact email	mathieu.brochet@unige.ch
lab head
alexandre Hainard
contact affiliation	University of Geneva
contact email	alexandre.hainard@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017308
     1. Label: PRIDE project
     2. Name: A divergent cyclin/cyclin-dependent kinase complex controls progression through the atypical replicative cycles during Plasmodium berghei gametogony