PXD017305

PXD017305 is an original dataset announced via ProteomeXchange.

Title	Impact of a conjugative plasmid on plant growth-promoting Bacillus velezensis
Description	Bacillus velezensis strain GH1-13 with a native conjugative plasmid (pBV71) is thought to be beneficial to the bacterium, although no information on its effects exists. Here we show that strain GH1-13 frequently lost the plasmid during normal growth conditions in a rich medium and changed the morphology and sensitivity to selenite and tellurite. Compared to the plasmid-cured cells, the wild-type and complemented cells exhibited multicellular behavior with the expression of conjugative type IV pili and regulatory Rap homologous genes that regulate the interconnection between conjugation and biofilm formation. Further omics-based analyses of morphogenesis, biofilm formation, and antibiotic synthesis suggest that the conjugative plasmid activates envelope stress responses in association with increased biosynthesis of extracellular polysaccharide and antibiotics for protective functions of the host during exponential phase.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:22:02.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong-Hak Kim
SpeciesList	scientific name: Bacillus velezensis; NCBI TaxID: 492670;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-28 06:28:36	ID requested
1	2021-05-17 04:26:08	announced
⏵ 2	2024-10-22 05:22:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: plant growth-promoting rhizobacteria, conjugative plasmid,Bacillus velezensis

Yong-Hak Kim
contact affiliation	Daegu Catholic University School of Medicine
contact email	ykim@cu.ac.kr
lab head
Yong-Hak Kim
contact affiliation	Catholic University of Daegu School of Medicine
contact email	ykim@cu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017305
     1. Label: PRIDE project
     2. Name: Impact of a conjugative plasmid on plant growth-promoting Bacillus velezensis