PXD017289

PXD017289 is an original dataset announced via ProteomeXchange.

Title	Dpb11 Immuno-Precipitation Mass Spectrometry (IPMS) Data Related to Mec1/ATR Signaling Upon Hyper-Resection
Description	The Mec1/ATR kinase is crucial for genome maintenance, although the mechanisms by which it controls DNA repair pathways remain elusive. Here we uncovered a role for Mec1/ATR in controlling homologous recombination (HR) factors in response to hyper-resection of DNA ends. Cells lacking RAD9, a checkpoint activator and an inhibitor of resection, exhibit Mec1-dependent hyper-phosphorylation of proteins associated with single strand DNA transactions, including the ssDNA binding protein Rfa2, the translocase/ubiquitin ligase Uls1 and the HR-regulatory Sgs1-Top3-Rmi1 (STR) complex. Extensive Mec1-dependent phosphorylation of the STR complex, mostly on the Sgs1 helicase subunit, promotes an interaction between STR and the DNA repair scaffolding protein Dpb11. Fusion of Sgs1 to phosphopeptide-binding domains of Dpb11 strongly impairs HR-mediated repair, supporting a model whereby Mec1 promotes recruitment of STR to the 9-1-1 clamp via Dpb11 to regulate recombinogenic processes.
HostingRepository	PRIDE
AnnounceDate	2021-03-10
AnnouncementXML	Submission_2021-03-10_08:21:16.806.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ethan Sanford
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-27 02:22:01	ID requested
⏵ 1	2021-03-10 08:21:17	announced

Dataset with its publication pending

submitter keyword: Yeast, APMS, DNA Repair, DNA Damage, Dpb11

Marcus Smolka
contact affiliation	Department of Molecular Biology and Genetics, Cornell University, USA
contact email	mbs266@cornell.edu
lab head
Ethan Sanford
contact affiliation	Graduate Student, Smolka Lab, Cornell University
contact email	ejs372@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017289
     1. Label: PRIDE project
     2. Name: Dpb11 Immuno-Precipitation Mass Spectrometry (IPMS) Data Related to Mec1/ATR Signaling Upon Hyper-Resection