PXD017285

PXD017285 is an original dataset announced via ProteomeXchange.

Title	Exposure of THP1 cells to plasma containing or lacking cfDNA
Description	We performed all stimulatory experiments using THP1 cell line as a representative of primary human monocytes to show fundamental role of the cfDNA in healthy organisms. The experiments were conducted in duplicates using plasma containing cfDNA (NP) and the reference one with cfDNA removed by DNase (TP) to recognize unequivocally the effect of plasma cfDNA on transcriptome and proteome of monocytes. We used native human plasma samples obtained from healthy volunteers with no animal serum addition to cultivation medium in order to avoid the presence of uncharacterized animal cfDNA in the experiments.
HostingRepository	PRIDE
AnnounceDate	2021-05-19
AnnouncementXML	Submission_2021-05-19_01:22:38.154.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017285
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-27 01:26:41	ID requested
⏵ 1	2021-05-19 01:22:39	announced

Korabecna M, Zinkova A, Brynychova I, Chylikova B, Prikryl P, Sedova L, Neuzil P, Seda O, Cell-free DNA in plasma as an essential immune system regulator. Sci Rep, 10(1):17478(2020) [pubmed]

submitter keyword: THP1 cells, plasma, cfDNA

Marie Korabecna
contact affiliation	Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
contact email	Marie.Korabecna@lf1.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD017285

PRIDE project URI

• PRIDE
  1. PXD017285
     1. Label: PRIDE project
     2. Name: Exposure of THP1 cells to plasma containing or lacking cfDNA