PXD017281

PXD017281 is an original dataset announced via ProteomeXchange.

Title	Parallel reaction monitoring assay development for multiple sclerosis biomarkers in cerebrospinal fluid.
Description	We have developed and tested parallel reaction monitoring (PRM) assays for 25 proteins highly relevant as cerebrospinal fluid (CSF) biomarkers for multiple sclerosis, representing various biological processes affected in the disease. The proteins were represented by 72 peptides selected according to relevant guidelines and available literature. Stability testing revealed 64 peptides with low intra- and inter-day variations, with 44 also being stably digested after 16 hours of trypsin digestion, and 37 furthermore showing a significant difference between multiple sclerosis and controls, thereby confirming literature findings. Calibration curves were developed using spike-in of synthetic light and heavy peptides in rat plasma.
HostingRepository	PanoramaPublic
AnnounceDate	2021-05-20
AnnouncementXML	Submission_2021-05-20_10:27:43.543.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Astrid Guldbrandsen
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-24 11:26:26	ID requested
⏵ 1	2021-05-20 10:27:44	announced

Guldbrandsen A, Lereim RR, Jacobsen M, Garberg H, Kroksveen AC, Barsnes H, Berven FS, Development of robust targeted proteomics assays for cerebrospinal fluid biomarkers in multiple sclerosis. Clin Proteomics, 17():33(2020) [pubmed]

submitter keyword: parallel reaction monitoring, cerebrospinal fluid, multiple sclerosis, neurological diseases, proteomics, biomarker

Frode Berven
contact affiliation	Proteomics Unit (PROBE), Department of Biomedicine, University of Bergen, Norway
contact email	fberven76@gmail.com
lab head
Astrid Guldbrandsen
contact affiliation	Proteomics Unit (PROBE), Department of Biomedicine, University of Bergen, Norway
contact email	astrid.guldbrandsen@uib.no
dataset submitter

Panorama Public dataset URI