PXD017280

PXD017280 is an original dataset announced via ProteomeXchange.

Title	A mouse brain-based multi-omics integrative approach reveals potential blood biomarkers for ischemic stroke
Description	Stroke remains a leading cause of death and disability worldwide. Despite continuous advances, the identification of key molecular signatures of acute ischemic stroke is still of primary interest for a real translational research on stroke diagnosis, prognosis and treatment. Integration of data from high-throughput -omics techniques is crucial to unravel key interactions among different molecular elements in a complex biological context. Here, we used advanced data integration methods for a multi-level joint analysis of transcriptomics and proteomics datasets depicted from the mouse brain 2h after cerebral ischemia. We identified a correlation-based integrated network of genes and proteins differentially expressed acutely after stroke. Of those, CLDN20, GADD45G, RGS2, BAG5 and CTNND2 were further evaluated as blood biomarkers of cerebral ischemia in mice and human blood samples. Our findings indicated that CTNND2 and GADD45G levels in blood within the first hours after ischemic stroke might be potentially useful to discriminate ischemic strokes from stroke-mimicking conditions and to predict stroke patients’ poor outcome, respectively. Together, we used for the first time an integrative biostatistical approach to elucidate key molecules of the initial stages of stroke pathophysiology and highlight new outstanding proteins that might be further considered as blood biomarkers of ischemic stroke.
HostingRepository	PanoramaPublic
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-07_07:02:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laura Ramiro
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-24 09:55:26	ID requested
⏵ 1	2020-09-07 07:02:59	announced

Simats A, Ramiro L, Garc, í, a-Berrocoso T, Brians, ó F, Gonzalo R, Mart, í, n L, Sab, é A, Gill N, Penalba A, Colom, é N, S, á, nchez A, Canals F, Bustamante A, Rosell A, Montaner J, A Mouse Brain-based Multi-omics Integrative Approach Reveals Potential Blood Biomarkers for Ischemic Stroke. Mol Cell Proteomics, 19(12):1921-1936(2020) [pubmed]

submitter keyword: ischemic stroke

proteomics

transcriptomics

integrative analysis

biomarkers

Joan Montaner
contact affiliation	Neurovascular Research Laboratory, Vall d’Hebron Institute of Research (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain
contact email	joan.montaner@vhir.org
lab head
Laura Ramiro
contact affiliation	Neurovascular Research Laboratory, Vall d’Hebron Institute of Research (VHIR)
contact email	laura.ramiro@vhir.org
dataset submitter

Panorama Public dataset URI