PXD017278

PXD017278 is an original dataset announced via ProteomeXchange.

Title	Factor Inhibiting HIF catalyses two asparaginyl-hydroxylations in VNVN motifs of ankyrin proteins
Description	The aspariginyl-hydroxylase activity of Factor Inhibiting HIF (FIH) is a key regulator of the transcriptional activity of the hypoxia inducible factor. FIH also catalyses the hydroxylation of asparaginyl- and other-residues in ankyrin repeat domain (ARD) containing proteins, including Apoptosis stimulating of p53 (ASPP) protein family members. ASPP2 is reported to undergo a single FIH catalysed hydroxylation at Asn-986. We report biochemical and crystallographic evidence showing FIH can catalyse the unprecedented post-translational hydroxylation of both asparaginyl-residues in “VNVN” and related motifs of ankyrin repeat domains in apoptosis-stimulating of p53 (ASPP) proteins (i.e. ASPP1, ASPP2 and iASPP) and the related ASB11 and p18-INK4C proteins. The results extend the substrate scope of FIH catalysis and may have implications for its role in the hypoxic response and, ASPP protein function.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:39:37.212.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017278
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Roman Fischer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	GG; Bacillosamine; GluGlu; Hex; Bromo; Methylphosphonate; Delta:H(4)C(2)O(-1)S(1); Propionamide; PhosphoHex; Deamidated; sulfo+amino; Trp->Oxolactone; Methylamine; Cation:K; Argbiotinhydrazide; Octanoyl; Glycosyl; Gln->pyro-Glu; Met->Hsl; Diethylphosphate; Guanidinyl; Menadione-HQ; DAET; NA-LNO2; HexNAc(2); Chlorination; Trimethyl; Fluoro; Ammonia-loss; ISD_z+2_ion; Oxidation; Piperidine; AEC-MAEC; Ethanedithiol; Carboxy; Met->Hpg; O-pinacolylmethylphosphonate; BITC; 4-ONE; PET; Quinone; Ethanolamine; Cation:Na; LG-lactam-R; maleimide3; Palmitoleyl; PyMIC; Trp->Hydroxykynurenin; Sulfo; MG-H1; Deoxy; BEMAD_ST; Malonyl; Methyl+Deamidated; Formyl; glucosone; Carbamyl; Arg->Orn; LG-anhydrolactam; Cation:Mg[II]; 3-deoxyglucosone; Sulfide; NDA; Amino; Ammonium; Lipoyl; Pro->Pyrrolidone; Crotonaldehyde; Met->Hse; Dethiomethyl; Amidated; Diethyl; Lys->AminoadipicAcid; Pro->pyro-Glu; Ethanolyl; Phospho; Hep; PEITC; Propargylamine; Hex(1)HexNAc(1); Acetyl; Palmitoyl; Nitro; Methylthio; Carboxymethyl; Cation:Ag; HexNAc; Carbamidomethyl; Cation:Ca[II]; Biotin-PEO-Amine; cGMP+RMP-loss; trifluoro; Puromycin; Glu->pyro-Glu; Carboxyethyl; MercaptoEthanol; Hydroxymethyl; Hex(1)HexNAc(1)NeuAc(1); dHex; Delta:H(2)C(2); Ethyl; Cation:Li; O-Isopropylmethylphosphonate; Cation:Fe[II]; PyruvicAcidIminyl; Isopropylphospho; AEBS; Pyridylacetyl; Dihydroxyimidazolidine; Methylmalonylation; Glucosylgalactosyl; Phosphopropargyl; HNE-Delta:H(2)O; Delta:H(4)C(3); TNBS; DEDGFLYMVYASQETFG; Didehydro; Cytopiloyne+water; Trp->Kynurenin; Glu; Pro->Pyrrolidinone; Arg->GluSA; Dansyl; Iminobiotin; SMA; Iodo; Hex(3)HexNAc(1)Pent(1); HexN; Diisopropylphosphate; Nmethylmaleimide; DimethylpyrroleAdduct; Lys->Allysine; LG-anhyropyrrole; Amidine; ethylamino; Gly-loss+Amide; maleimide; O-Methylphosphate; Dehydrated; Biotin; Dioxidation; Ethyl+Deamidated; Xlink:DTSSP[88]; Benzoyl; Carbofuran; glycidamide
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-24 06:28:03	ID requested
⏵ 1	2024-10-08 11:39:42	announced

10.6019/PXD017278;

10.1016/j.jbc.2022.102020;

Leissing TM, Hardy AP, Chan H, Wang Y, Tumber A, Chowdhury R, Feng T, Coleman ML, Cockman ME, Kramer HB, Berridge G, Fischer R, Kessler BM, Ratcliffe PJ, Lu X, Schofield CJ, Factor inhibiting HIF can catalyze two asparaginyl hydroxylations in VNVN motifs of ankyrin fold proteins. J Biol Chem, 298(6):102020(2022) [pubmed]

submitter keyword: Hydroxylation, HIF,ASPP

Roman Fischer
contact affiliation	Head of Discovery Proteomics Facility
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Roman Fischer
contact affiliation	University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017278
     1. Label: PRIDE project
     2. Name: Factor Inhibiting HIF catalyses two asparaginyl-hydroxylations in VNVN motifs of ankyrin proteins