PXD017273

PXD017273 is an original dataset announced via ProteomeXchange.

Title	Human and mouse brain, and human cerebrospinal fluid extracellular vesicles LC-MSMS
Description	Extracellular vesicles (EVs) are a unified terminology of membrane-enclosed vesicular species ubiquitously secreted by almost every cell type and present in all body fluids. They carry a cargo of lipids, metabolites, nucleic acids and proteins for their clearance from cells as well as for cell-to-cell communications. The exact composition of EVs and their specific functions are not well understood due to the underdevelopment of the separation protocols, especially those from the central nervous system including animal and human brain tissues as well as cerebrospinal fluids. To understand their exact molecular composition and their functional roles, development of the reliable protocols for EV separation is necessary. Known EV separation methods include differential centrifugation-ultracentrifugation, sucrose gradient ultracentrifugation, size exclusion chromatography, ultra-filtration, microfluidics, high-resolution flow cytometric sorting, polymer-based precipitation, immunoaffinity capture, affinity capture, and asymmetric-flow field-flow fractionation. In this report, we describe our EV separation protocols from human biospecimens, such as unfixed frozen brain tissues and cerebrospinal fluids. We also discuss the quality control measures of the above separation protocols by biophysical and proteomic characterizations.
HostingRepository	PRIDE
AnnounceDate	2020-02-17
AnnouncementXML	Submission_2020-02-17_13:26:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Satoshi Muraoka
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-24 03:16:45	ID requested
⏵ 1	2020-02-17 13:26:17	announced

Muraoka S, Lin W, Chen M, Hersh SW, Emili A, Xia W, Ikezu T, Assessment of separation methods for extracellular vesicles from human and mouse brain tissues and human cerebrospinal fluids. Methods, 177():35-49(2020) [pubmed]

submitter keyword: Human, mouse, brain tissue, cerebrospinal fluid, extracellular vesicles, TMT label, LC-MSMS

Tsuneya Ikezu
contact affiliation	Departments of Pharmacology & Experimental Therapeutics and Neurology Boston University School of Medicine
contact email	tikezu@bu.edu
lab head
Satoshi Muraoka
contact affiliation	Boston University School of Medicine
contact email	smuraoka@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD017273

PRIDE project URI

• PRIDE
  1. PXD017273
     1. Label: PRIDE project
     2. Name: Human and mouse brain, and human cerebrospinal fluid extracellular vesicles LC-MSMS