PXD017270 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of palmitoyl protein thioesterase 1 substrates defines roles for synaptic depalmitoylation |
Description | We report here the identification of substrates of the depalmitoylating enzyme PPT1 by quantitative mass spectrometry. We utilized a stringent two-step Acyl Resin-Assisted Capture (Acyl RAC) screen in which increased palmitoylation in vivo in PPT1 knockout is the first selection criteria for substrates. We validated hits by direct depalmitoylation with PPT1 to identify bona fide substrates. Most proteins identified are previously reported to be palmitoylated and the few known PPT1 substrates were validated. Significantly, the screen identified >100 proteins not previously known to be depalmitoylated by PPT1, including a wide range of endocytic, signaling, synaptic adhesion, mitochondrial, and lysosomal proteins. These proteins collectively explain the many facets of CLN1 disease caused by the loss of PPT1 function. We determined that an Ig domain in synaptic adhesion molecules may be a PPT1 recognition site for depalmitoylation. Finally, we provide evidence that PPT1 participates in neuronal activity-dependent palmitoylation-depalmitoylation cycles at the synapse. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:36:25.665.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | TuKiet Lam |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-23 06:21:08 | ID requested | |
1 | 2022-05-19 06:00:30 | announced | |
⏵ 2 | 2024-10-22 05:36:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1371/journal.pbio.3001590; |
Gorenberg EL, Massaro Tieze S, Y, ΓΌ, cel B, Zhao HR, Chou V, Wirak GS, Tomita S, Lam TT, Chandra SS, highlights roles of depalmitoylation in disulfide bond formation and synaptic function. PLoS Biol, 20(3):e3001590(2022) [pubmed] |
Keyword List
submitter keyword: synapse, synaptic cleft, synaptic vesicle endocytosis, neuronal ceroid lipofuscinoisis, synaptic adhesion molecules, AMPA receptor, neurodegeneration,palmitoylation, Palmitoyl protein thioesterase 1 |
Contact List
TuKiet Lam |
contact affiliation | Department of Molecular Biophysics and Biochemistry, Yale University, Keck MS & Proteomics Resource at Yale. |
contact email | tukiet.lam@yale.edu |
lab head | |
TuKiet Lam |
contact affiliation | Yale University |
contact email | tukiet.lam@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD017270 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017270
- Label: PRIDE project
- Name: Identification of palmitoyl protein thioesterase 1 substrates defines roles for synaptic depalmitoylation