PXD017268

PXD017268 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium
Description	The oral mucosal pellicle is a thin lubricating layer generated by the binding of saliva proteins on epithelial oral cells. The protein composition of this biological structure has been to date studied by targeted analyses of specific salivary proteins. In order to perform a more exhaustive proteome characterization of pellicles, we used TR146 cells expressing or not the transmembrane mucin MUC1 and generated pellicles by incubation with human saliva and washing to remove unbound proteins. A suitable method was established for the in vitro isolation of the mucosal pellicle by “shaving” it from the cells using trypsin. Comparison of pellicle and saliva compositions evidenced the adsorption of proteins not previously reported as pellicle constituents such as proteins of the PLUNC family. Pellicles formed on TR146 and TR146/MUC1 were also analyzed and compared by protein label-free quantification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:11.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gianluigi Cabiddu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-23 06:20:55	ID requested
1	2020-05-05 22:24:55	announced
⏵ 2	2024-10-22 05:03:12	announced	2024-10-22: Updated project metadata.

Cabiddu G, Maes P, Hyvrier F, Olianas A, Manconi B, Brignot H, Canon F, Cabras T, Morzel M, Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium. J Proteomics, 222():103797(2020) [pubmed]

10.1016/j.jprot.2020.103797;

submitter keyword: saliva, cellular model of oral mucosa, TR146/MUC1 cells, mucosal pellicle

Gianluigi Cabiddu
contact affiliation	University of Cagliari
contact email	cabgianluigi@gmail.com
lab head
Gianluigi Cabiddu
contact affiliation	University of Cagliari
contact email	cabgianluigi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017268
     1. Label: PRIDE project
     2. Name: Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium