PXD017267

PXD017267 is an original dataset announced via ProteomeXchange.

Title	Ribosomal and tRNA Biogenesis Interact to Control Hypoxic Injury and Translation
Description	Protein translation is an essential cellular process, but paradoxically, it can also promote aging and other stresses. In a screen for mutations that protect C. elegans from hypoxic stress, we isolated multiple genes that impact translation, including a ribosomal RNA helicase gene, ddx-52, tRNA biosynthesis genes, and a gene controlling amino acid availability. To better define the mechanisms whereby these genes control hypoxic injury, we performed a second screen for genetic suppressors of the ddx-52(lf) phenotypes. This screen identified multiple genes involved in ribosome biogenesis. Surprisingly, the suppressor mutations were able to restore normal hypoxic sensitivity and protein synthesis to the tRNA biosynthesis mutants, but not to the mutant reducing amino acid uptake. Proteomic characterization of the mutants demonstrated that reduced tRNA biosynthetic activity produces a corresponding reduction in ribosomal structural subunits. Our study uncovers an uncharacterized regulatory interaction between ribosomal biogenesis and tRNA abundance controlling translation and hypoxic survival. A proteomic analysis of a threonyl-tRNA synthetase mutant with and without the ribosomal biogenesis suppressors revealed an uncharacterized feedback mechanism whereby disruption of tRNA aminoacylation results in a corresponding reduction in ribosomal subunits, thereby explaining the ability of enhanced ribosomal biogenesis to suppress reduced tRNA aminoacylation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:31:54.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoting Tang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-23 06:20:42	ID requested
1	2022-02-15 11:07:32	announced
⏵ 2	2024-10-22 05:32:02	announced	2024-10-22: Updated project metadata.

Itani OA, Zhong X, Tang X, Scott BA, Yan JY, Flibotte S, Lim Y, Hsieh AC, Bruce JE, Van Gilst M, Crowder CM, Coordinate Regulation of Ribosome and tRNA Biogenesis Controls Hypoxic Injury and Translation. Curr Biol, 31(1):128-137.e5(2021) [pubmed]

10.1016/j.cub.2020.10.001;

submitter keyword: Ribosomal biogenesis, SILAC analysis of protein synthesis rate, LFQ and TMT quantification, hypoxic sensitivity

James E Bruce
contact affiliation	Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA
contact email	jimbruce@uw.edu
lab head
Xiaoting Tang
contact affiliation	University of Washington
contact email	xiaoting@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD017267

PRIDE project URI

• PRIDE
  1. PXD017267
     1. Label: PRIDE project
     2. Name: Ribosomal and tRNA Biogenesis Interact to Control Hypoxic Injury and Translation