PXD017262

PXD017262 is an original dataset announced via ProteomeXchange.

Title	Label-free Proteomics for Discovering Biomarker Candidates for Controlling Krypton Misuse in Castrated Horses (Geldings)
Description	Recent advances in label-free quantitative proteomics may support its application in identifying and monitoring biomarkers for the purpose of doping control in equine sports. In this study, we developed a workflow of label-free quantitative proteomics to propose plasma protein biomarkers in horses after administration with krypton (Kr), a potential erythropoiesis-stimulating agent. Plasma proteomes were profiled by using nano-liquid chromatography-high resolution mass spectrometry. An in-house mass spectral library consisting of 1,121 proteins was compiled using samples collected from geldings (castrated horses) in the administration trial and geldings in training. A data-independent acquisition (DIA) method was used to quantify an array of plasma proteins across plasma samples from the administration trial. Statistical analyses proposed a profile of 83 biomarker candidates that successfully differentiated Kr-administered samples from control samples, with the ability to detect Kr exposure for up to 13 days (the last sample collected in the administration trial). The model also correctly classified 32 in-training geldings as untreated controls. This is significantly longer than the one hour detection time of plasma Kr using headspace gas chromatography-tandem mass spectrometry. Bioinformatic analyses enriched biomarker candidates relevant to complement activation and iron metabolism. The upregulation of transferrin receptor protein 1 (TFRC), one of the candidates related to iron metabolism, in plasma after Kr administration was validated by selected reaction monitoring (SRM) of corresponding peptides. These results have demonstrated label-free quantitative proteomics as a promising approach to propose plasma protein biomarkers to enhance doping control.
HostingRepository	PRIDE
AnnounceDate	2020-02-20
AnnouncementXML	Submission_2020-02-20_06:18:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kin-Sing WONG
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; TSQ Quantiva

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-23 06:20:14	ID requested
⏵ 1	2020-02-20 06:18:26	announced

Wong KS, Cheung HW, Choi TLS, Kwok WH, Curl P, Mechie SC, Prabhu A, Wan TSM, Ho ENM, Label-free Proteomics for Discovering Biomarker Candidates for Controlling Krypton Misuse in Castrated Horses (Geldings). J Proteome Res, 19(3):1196-1208(2020) [pubmed]

submitter keyword: proteomics, biomarkers, data-independent acquisition, krypton, horse

Dr Emmie N.M. Ho
contact affiliation	Racing Laboratory, The Hong Kong Jockey Club
contact email	emmie.nm.ho@hkjc.org.hk
lab head
Kin-Sing WONG
contact affiliation	Racing Laboratory, The Hong Kong Jockey Club
contact email	ks.wong@hkjc.org.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD017262

PRIDE project URI

• PRIDE
  1. PXD017262
     1. Label: PRIDE project
     2. Name: Label-free Proteomics for Discovering Biomarker Candidates for Controlling Krypton Misuse in Castrated Horses (Geldings)