PXD017251

PXD017251 is an original dataset announced via ProteomeXchange.

Title	Deep Characterization of the IFN-I/III-Stimulated Proteome in HBV-Transfected or Native HepG2 Cells
Description	To expand knowledge of the effects of interferon at the proteomic level, we treated HepG2 cells with IFN-alpha and IFN-lambda for 24 hours. HepG2.2.15 cells, a model for HBV infection, were also examined versus controls. MTT assays showed that optimized IFN levels (100 ng/ml) did not induce apoptosis relative to untreated controls. Including controls, more than 6,000 proteins were identified. Five replicates each of IFN-alpha treatment, IFN-lambda treatment, and control were performed, allowing confident identification of differentially expressed proteins. While a number of publications suggest that no interferon effect is evident upon HBV infection, our own results strongly suggest otherwise. Differential alterations of the proteasome were noted when comparing HBV infection against IFN-treatment. We also note that differential effects upon IFN treatment significantly overlapped with transcriptomic datasets when upregulation was examined. However, proteins downregulated upon IFN treatment show little overlap with these transcriptomic datasets.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:02.499.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Poorichaya Somparn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: HBV genotype D; NCBI TaxID: 489483;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-22 03:41:34	ID requested
1	2020-06-26 05:55:50	announced
2	2020-06-29 00:56:08	announced	2020-06-29: Updated publication reference for DOI(s): null.
3	2020-07-22 00:05:22	announced	2020-06-29: Updated publication reference for DOI(s): null. 2020-07-22: Updated publication reference for PubMed record(s): 32685848. 2020-07-22: Updated publication reference for DOI(s): null.
⏵ 4	2023-11-14 09:01:03	announced	2023-11-14: Updated project metadata.

Hodge K, Makjaroen J, Robinson J, Khoomrung S, Pisitkun T, Deep Proteomic Deconvolution of Interferons and HBV Transfection Effects on a Hepatoblastoma Cell Line. ACS Omega, 5(27):16796-16810(2020) [pubmed]

submitter keyword: Quantitative proteomics,Hepatitis B virus, Interferon alpha, Interferon lambda3

Trairak Pisitkun
contact affiliation	Center of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
contact email	trairak@gmail.com
lab head
Poorichaya Somparn
contact affiliation	Chulalongkorn university
contact email	poorichaya.s@chula.ac.th
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017251

PRIDE project URI

• PRIDE
  1. PXD017251
     1. Label: PRIDE project
     2. Name: Deep Characterization of the IFN-I/III-Stimulated Proteome in HBV-Transfected or Native HepG2 Cells