PXD017245

PXD017245 is an original dataset announced via ProteomeXchange.

Title	Molecular Profiling of Osteoclast Derived Apoptotic Bodies Reveals Extended Intercellular Regulations after Cell Death
Description	Increasing evidences are revealing the important biological functions of apoptotic bodies (ABs). Here we identified the proteomic profiles of ABs from osteoclasts of distinct differentiation stages and investigated the potential functions. ABs were isolated from apoptotic bone marrow macrophages (BMMs), pre-osteoclasts (pOCs) or mature osteoclasts (mOCs). Proteomic signature analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) followed by bioinformatic analysis showed that proteomic signatures of ABs exhibited high similarities with the parental cells. Functionally, pOC-ABs induced endothelial progenitor cell (EPC) differentiation and increased CD31hiEmcnhi endothelial cell formation in a murine bone defect model via PDGF-BB. mOC-ABs induced mesenchymal stem cell (MSC) osteogenic differentiation and promoted bone formation via RANKL reverse signaling. Together, our results mapped the detailed proteomic landscapes of osteoclast derived ABs and demonstrated that the biological functions of ABs are largely inherited from corresponding parental cells, suggesting a novel extended intercellular regulation after cell apoptosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:38.924.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qinyu Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-22 01:18:16	ID requested
1	2021-09-08 14:03:30	announced
⏵ 2	2024-10-22 05:26:42	announced	2024-10-22: Updated project metadata.

Ma Q, Liang M, Wu Y, Luo F, Ma Z, Dong S, Xu J, Dou C, Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res, 9(1):5(2021) [pubmed]

10.1038/s41413-020-00121-1;

submitter keyword: Molecular Profiling of Osteoclast Derived Apoptotic Bodies Reveals Extended Intercellular Regulations after Cell Death

Ce Dou
contact affiliation	Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
contact email	lance.douce@gmail.com
lab head
Qinyu Ma
contact affiliation	Third Military Medical University
contact email	mqy32473069@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD017245

PRIDE project URI

• PRIDE
  1. PXD017245
     1. Label: PRIDE project
     2. Name: Molecular Profiling of Osteoclast Derived Apoptotic Bodies Reveals Extended Intercellular Regulations after Cell Death