PXD017243

PXD017243 is an original dataset announced via ProteomeXchange.

Title	Impact of Staphylococcus aureus infection on the late lactation goat milk proteome: new perspectives for monitoring and understanding mastitis in dairy goats
Description	This investigation reports a differential proteomic analysis of the goat milk to evaluate and understand the protein changes induced by Staphylococcus spp. during a subclinical intramammary infection (IMI). A number of 9 milk samples from multiparous goats were selected for the study, 3 of which in mid-lactation (30-60 Days in milk - DIM) with very low somatic cell count (SCC) from half-udders producing a sterile milk bacterial culture (MLU), 3 of which in late lactation samples (> 250 DIM) with SCC > 2,000,000 cells/mL from half-udders producing a sterile milk bacterial culture for the whole lactation (LHU) and in conclusion 3 in late lactation (> 250 DIM) with SCC > 2,000,000 cells/mL from goat half-udders with a milk bacterial culture repeatedly positive for Staphylococcus aureus (LHS). Samples were analyzed using a shotgun proteomics approach, based on filter-aided sample preparation (FASP) followed by LC-MS/MS and on differential analysis conducted by spectral count approach (1). (1) Pisanu S, Cacciotto C, Pagnozzi D, Puggioni GMG, Uzzau S, Ciaramella P, Guccione J, Penati M, Pollera C, Moroni P, Bronzo V, Addis MF. Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci. Sci Rep, 2019, 9 (1), 15850. Here, we reported both a characterization of goat milk proteins and a panel of differential proteins specific of S. Aureus-infected milk that mostly affected proteins involved in defense response processes and cytoskeleton organization. In conclusion, our results provide a depth characterization of milk proteins in goat samples uninfected and infected with S. Aureus, describe the changes induced by LHU and LHS subclinical intramammary infection and suggest indications to reveal subclinical staphylococcal mastitis in goat by a proteomic investigation of milk.
HostingRepository	PRIDE
AnnounceDate	2020-04-09
AnnouncementXML	Submission_2020-04-30_03:36:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salvatore Pisanu
SpeciesList	scientific name: Capra hircus; NCBI TaxID: 9925;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-22 01:17:58	ID requested
1	2020-04-09 08:47:21	announced
⏵ 2	2020-04-30 03:48:54	announced	2020-04-30: Updated publication reference for PubMed record(s): 32275959.

Pisanu S, Cacciotto C, Pagnozzi D, Uzzau S, Pollera C, Penati M, Bronzo V, Addis MF, Impact of Staphylococcus aureus infection on the late lactation goat milk proteome: New perspectives for monitoring and understanding mastitis in dairy goats. J Proteomics, 221():103763(2020) [pubmed]

submitter keyword: Biological dataset, Capra hircus, milk, mastitis, Label-free proteomics, LC-MS/MS

Daniela Pagnozzi
contact affiliation	Porto Conte Ricerche, Alghero, Sardinia, Italy
contact email	pagnozzi@portocontericerche.it
lab head
Salvatore Pisanu
contact affiliation	Porto Conte Ricerche, Alghero, Sardinia, Italy.
contact email	pisanu@portocontericerche.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017243
     1. Label: PRIDE project
     2. Name: Impact of Staphylococcus aureus infection on the late lactation goat milk proteome: new perspectives for monitoring and understanding mastitis in dairy goats