PXD017242

PXD017242 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Functionalized Peptidisc Scaffolds Enable Affinity Purification of the Membrane Proteome
Description	We reconstituted the total E. coli membrane proteome into His-tagged peptidiscs. We then performed an affinity purification to enrich the bona fide membrane proteome away from soluble contaminants which co-sediment with cellular membranes after cell lysis. As a case study, we employ this method to survey changes to the membrane proteome caused by altered gene expression.
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_03:27:06.916.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Rattray
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-22 01:17:57	ID requested
⏵ 1	2022-04-13 03:27:07	announced

Publication List

Young JW, Wason IS, Zhao Z, Rattray DG, Foster LJ, Duong Van Hoa F, His-Tagged Peptidiscs Enable Affinity Purification of the Membrane Proteome for Downstream Mass Spectrometry Analysis. J Proteome Res, 19(7):2553-2562(2020) [pubmed]

Young JW, Wason IS, Zhao Z, Rattray DG, Foster LJ, Duong Van Hoa F, His-Tagged Peptidiscs Enable Affinity Purification of the Membrane Proteome for Downstream Mass Spectrometry Analysis. J Proteome Res, 19(7):2553-2562(2020) [pubmed]

Keyword List

submitter keyword: Peptidisc, E. coli, Membrane interactome, Membrane protein reconstitution

submitter keyword: Peptidisc, E. coli, Membrane interactome, Membrane protein reconstitution

Contact List

Franck Van Hoa Duong
contact affiliation	Department of Biochemistry and Molecular Biology, University of British Columbia, Canada.
contact email	fduong@mail.ubc.ca
lab head
David Rattray
contact affiliation	University of British Columbia
contact email	rattrayd@msl.ubc.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD017242
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD017242

PRIDE project URI

Repository Record List

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