PXD017241

PXD017241 is an original dataset announced via ProteomeXchange.

Title	Definition of the fibroblast adhesome using multiplexed proximity biotinylation
Description	Integrin adhesion complexes (IACs) bridge the extracellular matrix to the actin cytoskeleton and transduce signals in response to both chemical and mechanical cues. The composition, interactions, stoichiometry and topological organisation of proteins within IACs are not fully understood. To address this gap, we used multiplexed proximity biotinylation (BioID) to generate an in situ, proximity-dependent adhesome. Integration of the interactomes of 16 IAC-associated baits revealed a network of 147 proteins with 361 proximity interactions. Candidates with underappreciated roles in adhesion were identified, in addition to established IAC components. Bioinformatic analysis revealed five clusters of IAC baits that link to common groups of prey, and which therefore may represent functional modules. The five clusters, and their spatial associations, are consistent with current models of IAC interaction networks and stratification. This study provides a resource to examine proximal relationships within IACs on a global level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:55.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Craig Lawless
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-22 01:17:52	ID requested
1	2020-06-01 14:40:56	announced
2	2020-06-30 04:02:15	announced	2020-06-30: Updated publication reference for PubMed record(s): 32585685.
⏵ 3	2023-11-14 09:00:55	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: fibroblast, adhesome, BioID

Martin J Humphries
contact affiliation	Professor of Biochemistry, Faculty of Biology, Medicine & Health, Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, United Kingdom (lab head)
contact email	martin.humphries@manchester.ac.uk
lab head
Craig Lawless
contact affiliation	University of Manchester
contact email	craig.lawless@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017241

PRIDE project URI

• PRIDE
  1. PXD017241
     1. Label: PRIDE project
     2. Name: Definition of the fibroblast adhesome using multiplexed proximity biotinylation