PXD017238

PXD017238 is an original dataset announced via ProteomeXchange.

Title	Structural Basis for CAL1-Mediated Centromere Maintenance
Description	Intermolecular interactions stabilising the CAL1–CENP-A/H4 complex were analysed using chemical Cross-Linking Mass Spectrometry (CLMS). Purified recombinant CAL11-160–CENP-A101-225–H4 complex was crosslinked using EDC and BS3. The crosslinked peptides were analysed by mass spectrometry to identify intra- and intermolecular contacts. Notably, the data revealed several intramolecular crosslinks between the N- and C-terminal regions of CAL11-160, suggesting a direct interaction between these regions.
HostingRepository	PRIDE
AnnounceDate	2020-03-11
AnnouncementXML	Submission_2020-03-10_23:48:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-21 08:01:08	ID requested
⏵ 1	2020-03-10 23:48:06	announced

Medina-Pritchard B, Lazou V, Zou J, Byron O, Abad MA, Rappsilber J, Heun P, Jeyaprakash AA, Structural basis for centromere maintenance by Drosophila CENP-A chaperone CAL1. EMBO J, 39(7):e103234(2020) [pubmed]

submitter keyword: CAL1/CENP-A/CENP-C/centromeres/chromosome segregation

Juri Rappsilber
contact affiliation	Wellcome Centre for Cell Biology, University of Edinburgh Institute of Biotechnology, Technische Universität Berlin
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD017238

PRIDE project URI

• PRIDE
  1. PXD017238
     1. Label: PRIDE project
     2. Name: Structural Basis for CAL1-Mediated Centromere Maintenance