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PXD017233

PXD017233 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecond messenger control of mRNA translation by dynamic ribosome modification
DescriptionPseudomonas species employ complex, dynamic signalling networks to fine-tune responses to changing environments, with regulation taking place at the transcriptional, post-transcriptional and post-translational levels. Control of mRNA translation and hence protein abundance is a crucial element of this regulatory network. Previously, we identified the ribosomal modification protein RimK, which influences the transition between active and sessile bacterial lifestyles. RimK is an ATP-dependent glutamyl ligase that adds glutamate residues to the C-terminus of ribosomal protein RpsF. This in-turn induces specific changes in ribosome function and translational output. RimK activity is itself under complex, multifactorial control: by the bacterial second messenger cyclic-di-GMP; a phosphodiesterase trigger enzyme (RimA); and a polyglutamate-specific protease (RimB). Deletion of the rim operon affects phenotypes including attachment, motility and cytotoxicity, severely compromising rhizosphere colonisation by the soil bacterium Pseudomonas fluorescens. Using a combination of protein biochemistry, quantitative proteomics and ribosomal profiling experiments, we examined the relationship between ribosomal modification and downstream changes in the P. fluorescens proteome. RimK activity leads to active proteome remodelling by two main routes; indirectly, through changes in the abundance of the global translational regulator Hfq and directly, with translation of surface attachment factors, amino acid transporters and key secreted molecules linked specifically to RpsF modification. Our findings suggest post-translational ribosomal modification as a rapid-response mechanism to tune global gene translation and thereby protein abundance in response to environmental signals.
HostingRepositoryPRIDE
AnnounceDate2020-06-30
AnnouncementXMLSubmission_2020-06-30_04:00:59.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGerhard Saalbach
SpeciesList scientific name: Pseudomonas fluorescens SBW25; NCBI TaxID: 216595;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-21 05:57:29ID requested
12020-06-30 04:01:00announced
Publication List
Grenga L, Little RH, Chandra G, Woodcock SD, Saalbach G, Morris RJ, Malone JG, Control of mRNA translation by dynamic ribosome modification. PLoS Genet, 16(6):e1008837(2020) [pubmed]
Keyword List
submitter keyword: Pseudomonas, ribosomal proteins, signalling, cyclic-di-GMP, iTRAQ proteome
Contact List
Jacob George Malone
contact affiliationJohn Innes Centre / University of East Anglia
contact emailjacob.malone@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliationBiological Chemistry
contact emailgerhard.saalbach@jic.ac.uk
dataset submitter
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